Basic and acidic fibroblast growth factors modulate the epidermal growth factor receptor by a protein kinase C-independent pathway.
Basic and acidic fibroblast growth factors modulate the epidermal growth factor receptor by a protein kinase C-independent pathway.
复制标题
碱性和酸性成纤维细胞生长因子通过不依赖于蛋白激酶 C 的途径调节表皮生长因子受体。
DOI：
10.1016/0006-291x(89)91529-5
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发表时间：
1989
影响因子：
3.1
通讯作者：
Rosner,MR
中科院分区：
文献类型：
--
作者：
Hicks,K;Friedman,B;Rosner,MR
人酸性和碱性成纤维细胞生长因子（aFGF和bFGF）抑制小鼠Swiss 3 T3细胞中表皮生长因子（EGF）受体结合。Scatchard分析表明，aFGF和bFGF导致高亲和力EGF受体群体的减少，类似于蛋白激酶C的激活剂如佛波醇酯、血小板衍生生长因子（PDGF）和蛙皮素所观察到的。然而，与佛波醇酯不同，aFGF和bFGF抑制蛋白激酶C缺陷细胞中的EGF结合。aFGF和bFGF抑制EGF结合的时间过程和剂量反应非常相似，ID 50约为0.10 ng/ml。与铃蟾肽相反，但与PDGF相似，aFGF和bFGF均不通过百日咳毒素不敏感的G蛋白作用于EGF受体。这些结果表明，酸性和碱性FGF通过蛋白激酶CGi非依赖性途径以相似的效力抑制Swiss 3 T3细胞中的高亲和力EGF结合。
Human acidic and basic fibroblast growth factors (aFGF and bFGF) inhibit epidermal growth factor (EGF) receptor binding in mouse Swiss 3T3 cells. Scatchard analysis indicates that aFGF and bFGF cause a decrease in the high affinity EGF receptor population, similar to that observed for activators of protein kinase C such as phorbol esters, platelet-derived growth factor (PDGF) and bombesin. However, unlike phorbol esters, aFGF and bFGF inhibit EGF binding in protein kinase C-deficient cells. The time course and dose response of inhibition of EGF binding by both aFGF and bFGF are very similar, with an ID 50 of approximately 0.10 ng/ml. In contrast to bombesin but like PDGF, neither aFGF nor bFGF act on the EGF receptor through a pertussis toxinsensitive G protein. These results indicate that both acidic and basic FGF depress high affinity EGF binding in Swiss 3T3 cells with similar potency through a protein kinase C G i-independent pathway.