Enzyme encapsulation by protein cages.
Enzyme encapsulation by protein cages.
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蛋白质笼是具有纳米空腔的中空蛋白质壳，可以填充有用的材料。笼子的封装性质意味着它们对于装载生物大分子特别有吸引力，在生物大分子可能被降解的情况下为客人提供保护。鉴于蛋白质在工业和所有细胞过程中的重要性，功能性蛋白质货物（特别是酶）的封装对于体内诊断和治疗用途以及离体应用都引起高度关注。随着人们对高分辨率蛋白质笼结构的了解不断增加，以及人造蛋白质笼生产的最新进展，意味着它们现在可以在其内表面设计出各种附着化学物质——这是一种用于捕获货物的有用工具。在这里，我们回顾了最近已成功证明用于蛋白质笼中酶封装的不同可用附着策略，并考虑它们的未来潜力。蛋白质笼是有用的纳米容器，可以通过几种不同的方法捕获和封装酶。
Protein cages are hollow protein shells with a nanometric cavity that can be filled with useful materials. The encapsulating nature of the cages means that they are particularly attractive for loading with biological macromolecules, affording the guests protection in conditions where they may be degraded. Given the importance of proteins in both industrial and all cellular processes, encapsulation of functional protein cargoes, particularly enzymes, are of high interest both for in vivo diagnostic and therapeutic use as well as for ex vivo applications. Increasing knowledge of protein cage structures at high resolution along with recent advances in producing artificial protein cages means that they can now be designed with various attachment chemistries on their internal surfaces – a useful tool for cargo capture. Here we review the different available attachment strategies that have recently been successfully demonstrated for enzyme encapsulation in protein cages and consider their future potential. Protein cages are useful nanometric containers which can capture and encapsulate enzymes via several different approaches.
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