Improving sensitivity for microchip electrophoresis interfaced with inductively coupled plasma mass spectrometry using parallel multichannel separation.
Improving sensitivity for microchip electrophoresis interfaced with inductively coupled plasma mass spectrometry using parallel multichannel separation.
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DOI：
10.1016/j.chroma.2016.07.075
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发表时间：
2016-08
期刊：
影响因子：
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通讯作者：
Heyong Cheng;Jinhua Liu;Zigang Xu;Yuan-chao Wang;Meiying Ye
中科院分区：
文献类型：
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作者：
Heyong Cheng;Jinhua Liu;Zigang Xu;Yuan-chao Wang;Meiying Ye
本文报道了用多通道平行分离技术提高微芯片电泳与电感耦合等离子体质谱联用的灵敏度。通过使用2-20个阵列泳道进行平行分离，MCE-ICP-MS系统的灵敏度按比例提高了2-20倍。平行分离对柱效和分离度无明显影响。在800 V cm− 1的电场作用下，以5 mmol L− 1硼酸盐缓冲液（pH 9.2）为背景电解质，在2cm的20个通道中，Hg 2+和甲基汞离子在36 s内实现了快速分离。该系统对Hg 2+和甲基汞的检测限降低到6.8-7.1 ng L−1。标准鱼的测定值与标准值符合良好，回收率在94- 98%之间。结果表明，该方法具有灵敏度高、效率高、操作成本低等优点，可用于环境、生物、食品等领域中金属形态的常规分析。
We reported sensitivity enhancement using multichannel parallel separation for microchip electrophoresis hyphenated with inductively coupled plasma mass spectrometry (MCE-ICP-MS) in this study. By using 2-20 array lanes for parallel separation, the sensitivity of the MCE-ICP-MS system was proportionally improved by 2-20 folds. No significantly adverse effect of parallel separation on column efficiency and resolution was observed. Rapid separation of Hg2+and methylmercuric (MeHg) ion within 36 s under an electric field of 800 V cm−1was achieved in the 2-cm twenty-channels with a background electrolyte of 5 mmol L−1borate buffer (pH 9.2). Detection limits of Hg2+and MeHg by the proposed system were decreased to 6.8–7.1 ng L−1. Good agreement between determined values and certified values of a certified reference fish was obtained with recoveries ranged between 94–98%. All results prove its advantages including high sensitivity, high efficiency and low operation cost, which are beneficial to routine analysis of metal speciation in environmental, biological and food fields.