Internalization of human macrophage surface antigens induced by monoclonal antibodies
Internalization of human macrophage surface antigens induced by monoclonal antibodies
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DOI：
10.1016/0022-1759(95)00213-8
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发表时间：
1995-12-15
影响因子：
2.2
通讯作者：
Toujas, L
中科院分区：
文献类型：
--
作者：
Audran, R;Drenou, B;Toujas, L
预期被巨噬细胞内吞的药物可以通过针对这些细胞的单克隆抗体转运。为此目的研究了20种MAb。这些单克隆抗体与从血液单核细胞的7天培养物获得的巨噬细胞的结合显示，抗-CD 11b和抗-CD 14识别最多数量的细胞表面抗原位点。进一步的测定确定抗-CD 63、Mo 5和抗-CD 33是诱导相应抗原的最强调节的MAb，最高比率是抗-CD 63。细胞质中存在MAb证明了抗原-抗体复合物的内吞作用。在该测定中，抗CD 63 MAb诱导最高的内化。然而，对于大多数单克隆抗体，抗原位点的密度和抗原调节的强度不能预测在细胞质中检测到的单克隆抗体的量。
Drugs intended to be endocytosed by macrophages may be transported by MAbs directed against these cells. Twenty MAbs were investigated for this purpose. The binding of these MAbs to macrophages obtained from a 7 day culture of blood monocytes showed that anti-CD11b and anti-CD14 recognized the highest number of cell surface antigen sites. Further assays determined that anti-CD63, Mo5 and anti-CD33 were the MAbs that induced the strongest modulation of the corresponding antigens, the highest rate being with anti-CD63. Endocytosis of antigen-antibody complexes was evidenced by the presence of MAbs in the cytoplasm. Anti-CD63 MAbs induced the highest internalization in this assay. For most MAbs, however, the density of antigen sites and the intensity of antigen modulation were not predictive of the amount of MAb detected in the cytoplasm.