Fabrication and Characterization of Paper-Based Microfluidics Prepared in Nitrocellulose Membrane By Wax Printing
Fabrication and Characterization of Paper-Based Microfluidics Prepared in Nitrocellulose Membrane By Wax Printing
复制标题
蜡印硝化纤维素膜纸基微流控的制备和表征
DOI：
10.1021/ac9020193
复制
发表时间：
2010-01-01
影响因子：
7.4
通讯作者：
Lin, Bingcheng
中科院分区：
文献类型：
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作者：
Lu, Yao;Shi, Weiwei;Lin, Bingcheng
纸基微流体技术是一种很有前途的技术，可以为资源有限的环境开发一种简单、低成本、便携式和一次性的诊断平台。在这里，我们报告的纸基微流控器件的制作在硝酸纤维素膜蜡印刷蛋白质固定相关的应用。制作过程主要包括印刷和烘烤两个步骤，可在10 min内完成。蜡图案化将在膜中形成疏水区域，其可用于引导流动路径或分离反应区。优化了印刷方式和烘烤时间等制备参数，并对蜡图案化硝酸纤维素膜的印刷分辨率、蛋白质固定化和样品纯化能力等性能进行了表征。我们相信蜡图案化的硝酸纤维素膜将增强纸微流体器件的能力，并在该领域带来新的应用。
Paper-based microfluidics is a promising technology to develop a simple, low-cost, portable, and disposable diagnostic platform for resource-limited settings. Here we report the fabrication of paper-based microfluidic devices in nitrocellulose membrane by wax printing for protein immobilization related applications. The fabrication process, which can be finished within 10 min, includes mainly printing and baking steps. Wax patterning will form hydrophobic regions in the membrane, which can be used to direct the flow path or separate reaction zones. The fabrication parameters like printing mode and baking time were optimized, and performances of the wax-patterned nitrocellulose membrane such as printing resolution, protein immobilization, and sample purification capabilities were also characterized in this report. We believe the wax-patterned nitrocellulose membrane will enhance the capabilities of paper microfluidic devices and bring new applications in this field.