STIMULATORY EFFECT OF SHORT-CHAIN FATTY-ACIDS ON EPITHELIAL-CELL PROLIFERATION IN THE RAT INTESTINE - A POSSIBLE EXPLANATION FOR TROPHIC EFFECTS OF FERMENTABLE FIBER, GUT MICROBES AND LUMINAL TROPHIC FACTORS
STIMULATORY EFFECT OF SHORT-CHAIN FATTY-ACIDS ON EPITHELIAL-CELL PROLIFERATION IN THE RAT INTESTINE - A POSSIBLE EXPLANATION FOR TROPHIC EFFECTS OF FERMENTABLE FIBER, GUT MICROBES AND LUMINAL TROPHIC FACTORS
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DOI：
10.1079/bjn19870073
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发表时间：
1987-07-01
影响因子：
3.6
通讯作者：
SAKATA, T
中科院分区：
文献类型：
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作者：
SAKATA, T
1.研究了短链脂肪酸（SCFA）对回肠造瘘大鼠肠上皮细胞增殖的影响。2.每日剂量的乙酸、丙酸和正丁酸（分别为100、20和60 mM; pH 6.1）/瘘管（3 ml，每日2次）对隐窝细胞产生率（CCPR）的刺激作用在2 d内出现，并持续至少5 d。3.连续14天的SCFA日剂量使每日CCPR增加3至4倍。这种效应与肠道细菌的存在无关。4. SCFA的作用是剂量依赖性的，并且在酸之间变化（丁酸盐>丙酸盐>乙酸盐）。该效果是独立的低管腔pH值。相反，SCFA在体外抑制离体大鼠盲肠组织的上皮增殖。6.这些结果表明SCFA是由体内系统机制介导的生理性管腔营养因子。7.可以得出结论，SCFA参与肠道微生物的营养作用，可发酵纤维的摄入，和管腔内容物。
1. Effects of short-chain fatty acids (SCFA) on epithelial proliferation of the intestine were studied in ileally fistulated rats fed on an elemental diet. 2. The stimulatory effect of daily doses of acetic, propionic and n-butyric acids (100, 20 and 60 mM respectively; pH 6.1) per fistula (3 ml, twice daily) on crypt cell production rate (CCPR) appeared within 2 d and lasted for at least 5 d. 3. The daily doses of SCFA for 14 d increased daily CCPR three to four fold. This effect was independent of the presence of gut bacteria. 4. Effects of SCFA were dose-dependent and varied among acids (butyrate > propionate > acetate). The effect was independent of low lumen pH. 5. In contrast, SCFA inhibited epithelial proliferation of isolated rat caecal tissue in vitro. 6. These results suggest that SCFA are physiological lumen trophic factors mediated by a systemic mechanism in vivo. 7. It is concluded that SCFA are involved in the trophic effects of gut microbes, ingestion of fermentable fibre, and lumen contents.