Microarray analysis of genes showing variable expression following a blood meal in Anopheles gambiae
Microarray analysis of genes showing variable expression following a blood meal in Anopheles gambiae
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DOI：
10.1111/j.1365-2583.2005.00567.x
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发表时间：
2005-08-01
影响因子：
2.6
通讯作者：
Ribeiro, JMC
中科院分区：
文献类型：
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作者：
Marinotti, O;Nguyen, QK;Ribeiro, JMC
疟疾媒介蚊子冈比亚按蚊的14900个基因的微阵列分析表明，多达33%（4924）的相应的转录产物的丰度变化后24小时内的血液餐。这些产品中大约有一半（2388）的积累增加，其余的（2536）减少。80%的基因表达动态分析表达序列标签（EST）项目先前报道的是一致的观察，从这个微阵列分析。此外，微阵列分析在检测以低水平表达的基因产物的丰度的变化方面更灵敏，并且在检测更多数量的调节基因方面总体上更灵敏。转录丰度的主要变化见于编码参与消化、卵子发生和运动的蛋白质的基因。微阵列数据和所有表格的电子超链接版本可在http://www.angagepuci.bio.uci.edu/1/上向研究社区提供。
A microarray analysis of 14 900 genes of the malaria vector mosquito, Anopheles gambiae, shows that as many as 33% (4924) of their corresponding transcription products vary in abundance within 24 h after a blood meal. Approximately half (2388) of these products increase in their accumulation and the remainder (2536) decrease. Expression dynamics of 80% of the genes analysed by expressed sequence tag (EST) projects reported previously are consistent with the observations from this microarray analysis. Furthermore, the microarray analysis is more sensitive in detecting variation in abundance of gene products expressed at low levels and is more sensitive overall in that a greater number of regulated genes are detected. Major changes in transcript abundance were seen in genes encoding proteins involved in digestion, oogenesis and locomotion. The microarray data and an electronic hyperlinked version of all tables are available to the research community at http://www.angagepuci.bio.uci.edu/1/.