γ-tubulin complexes:: binding to the centrosome, regulation and microtubule nucleation
γ-tubulin complexes:: binding to the centrosome, regulation and microtubule nucleation
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DOI：
10.1016/s0955-0674(99)00064-2
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发表时间：
2000-02-01
影响因子：
7.5
通讯作者：
Schiebel, E
中科院分区：
文献类型：
--
作者：
Schiebel, E
微管组装在体内由γ-微管蛋白复合物启动。细胞质γ-微管蛋白复合物通过一组所谓的γ-微管蛋白复合物结合蛋白（GTBP）靶向中心体或其它微管组织中心（MTOC），所述GTBP可能与γ-微管蛋白复合物的保守的Spc 97 p/Spc 98 p蛋白家族相互作用。在其他细胞类型中，γ-微管蛋白复合物可以以MTOC非依赖性方式启动染色体附近的微管形成。最近，通过发现γ-微管蛋白、Spc 97 p和Spc 98 p形成可能负责微管成核的保守核心，以及通过发现酵母GTBP以细胞周期依赖性方式和响应于外部信号而被调节，已经取得了重大进展。此外，还发现小的GTdR Ran在其GDP结合状态下可以促进纺锤体组装。此外，γ-微管蛋白在草履虫基体复制中的重要功能已被证明。
Microtubule assembly is initiated in vivo by gamma-tubulin complexes. Cytoplasmic gamma-tubulin complexes are targeted to centrosomes or to other microtubule organizing centers (MTOCs) via a set of so called gamma-tubulin complex binding proteins (GTBPs) that probably interact with the conserved Spc97p/Spc98p protein family of gamma-tubulin complexes. In other cell types, gamma-tubulin complexes may initiate microtubule formation near chromosomes in a MTOC-independent manner. Recently, major advances have been achieved through the finding that gamma-tubulin, Spc97p and Spc98p form a conserved core that is probably responsible for microtubule nucleation, and by the discovery that a yeast GTBP is regulated in a cell-cycle-dependent manner and in response to an external signal. Furthermore, it was found that the small GTPase Ran in its GDP-bound state may promote spindle assembly. In addition, an essential function of gamma-tubulin in basal body duplication has been demonstrated in Paramecium.