Gold Nanoparticles and Hydrophobic Ionic Liquid Applied on the Development of a Voltammetric Biosensor for Polyphenol Determination
Gold Nanoparticles and Hydrophobic Ionic Liquid Applied on the Development of a Voltammetric Biosensor for Polyphenol Determination
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DOI：
10.1002/elan.201000732
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发表时间：
2011-05
期刊：
影响因子：
3
通讯作者：
E. Zapp;Daniela Brondani;I. Vieira;J. Dupont;C. Scheeren
中科院分区：
文献类型：
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作者：
E. Zapp;Daniela Brondani;I. Vieira;J. Dupont;C. Scheeren
以离子液体1-丁基-3-甲基咪唑六氟磷酸盐（Au-BMI. PF 6）为分散剂制备了纳米金纳米粒子和大蒜多酚氧化酶（PPO）生物传感器。采用方波伏安法测定了粘土固定化酶催化剂中多酚的含量。在优化条件下，分析曲线显示七叶酮浓度在0.40至9.86 µM（R=0.9997）范围内呈线性，检测限（LOD）为0.11 µM。该传感器具有良好的重复性和重现性，相对标准偏差分别为3.5%和4.6%.该方法已成功地应用于红茶样品中七叶壳含量的测定。
A novel biosensor based on gold nanoparticles dispersed in ionic liquid 1-butyl-3-methylimidazolium hexafluorophosphate (Au-BMI.PF6) and polyphenol oxidase (PPO) from garlic (Allium sativum L.) immobilized in clay was developed for determination of polyphenol by square-wave voltammetry. Under optimized conditions, the analytical curve showed a linear range for esculetin concentrations from 0.40 to 9.86 µM (R=0.9997), with a limit of detection (LOD) of 0.11 µM. This biosensor demonstrated good repeatability and reproducibility, with relative standard deviations of 3.5 % and 4.6 %, respectively. The method was successfully applied to the determination of esculetin content in black tea samples.