Fractionation of K-562 cells on the basis of their surface properties by partitioning in two-polymer aqueous-phase systems.
Fractionation of K-562 cells on the basis of their surface properties by partitioning in two-polymer aqueous-phase systems.
复制标题
通过在两种聚合物水相系统中分配，根据 K-562 细胞的表面特性进行分级。
DOI：
10.1007/bf02797342
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
Seaman,GV
中科院分区：
文献类型：
--
作者：
Walter,H;Al-Romaihi,FA;Krob,EJ;Seaman,GV
K-562细胞系是最初来源于处于急变期的慢性髓性白血病患者的人白血病干细胞的培养物。我们已经使这样的细胞在对数生长期在电荷敏感的葡聚糖-聚乙二醇水相系统中进行逆流分布，该方法基于细胞表面性质的细微差异对细胞进行分级，并发现：（1）细胞群体是异质的，因为它由具有不同分配比的细胞组成。(2)在增加细胞分配比和增加细胞电泳迁移率之间存在相关性。(3)在分布曲线的不同部分下的细胞在细胞周期的不同部分中具有不同的细胞比率，这种现象可能至少部分地是这些细胞亚分级的基础。随着分配比的增加，G 0 +G1+早期S中的细胞减少，而晚期S+G2+M中的细胞增加。(4)唾液酸是细胞的主要表面电荷组分，如用神经氨酸酶处理后其分配比的急剧下降所证明的。
The K-562 cell line is a culture of human leukemia stem cells originally derived from a patient with chronic myelogenous leukemia in blast crisis. We have subjected such cells, in the log phase of growth, to countercurrent distribution in a charge-sensitive dextran-polyethylene glycol aqueous-phase system, a method that fractionates cells on the basis of subtle differences in their surface properties, and found that: (1) The cell population is heterogeneous since it is composed of cells with different partition ratios. (2) There is a correlation between increasing cell partition ratios and increasing cell electrophoretic mobilities. (3) Cells under different parts of the distribution curve have dissimilar ratios of cells in different parts of the cell cycle, a phenomenon that may, at least partially, be the basis for the subfractionation of these cells. There is a clear tendency for cells in G0+G1+early S to decrease and for those in late S+G2+M to increase with increasing partition ratios. (4) Sialic acid is a major surface charge component of the cells as evidenced by a dramatic drop in their partition ratios after treatment with neuraminidase.