Molecular identification and in vitro antifungal susceptibility of blood isolates of the Candida parapsilosis species complex in Venezuela
Molecular identification and in vitro antifungal susceptibility of blood isolates of the Candida parapsilosis species complex in Venezuela
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DOI：
10.1016/j.riam.2016.11.005
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发表时间：
2017-07-31
影响因子：
1.9
通讯作者：
Dolande, Maribel
中科院分区：
文献类型：
--
作者：
Moreno, Xiomara;Reviakina, Vera;Dolande, Maribel
背景资料：近平滑念珠菌是由狭义近平滑念珠菌、近平滑念珠菌和偏平滑念珠菌组成的种复合体。目的：对近平滑假丝酵母菌（Candida parapsilosis）进行分子生物学鉴定，并测定其体外抗真菌药敏感性。方法：对86株近平滑假丝酵母菌（Candida parapsilosis）进行体外药敏试验。制备了2008-2011年收集的并从Rafael Rangel国家卫生研究所真菌学部门的真菌病监测网络获得的近平滑病物种复合体。使用聚合酶链反应进行仲醇脱氢酶基因扩增，并使用酶BanI通过限制性片段长度多态性分析产物。结果：86株分离株中，有81株（94.2%）为C.狭义近平滑病4例（4.6%）;直丝酵母菌1株（1.2%）;变形C.近平滑病分离株对阿替霉素B和卡泊芬净敏感，对氟康唑和伏立康唑耐药率低。C.直丝孢C.结论：在委内瑞拉获得的结果首次提供了关于C.在念珠菌血症的情况下，近平滑的物种复杂性，并支持需要继续监测计划，包括物种的分子鉴别和抗真菌药敏试验，这可能会指导特定的治疗。(C)2017 Asociacion Espanola de Micologia.出版社：Elsevier Espana，S.L.U. All rights reserved.
Background: Candida parapsilosis is a species complex consisting of Candida parapsilosis sensu stricto, Candida orthopsilosis, and Candida metapsilosis. Studies worldwide have described its epidemiology and susceptibility to antifungal agents.Aims: The aims of this study were to carry out the molecular identification of blood isolates belonging to the Candida parapsilosis species complex, and to determine their in vitro susceptibility to antifungals of systemic use.Methods: A study of 86 strains of C. parapsilosis species complex collected in 2008-2011 and obtained from the Candidaemia Surveillance Network of Mycology Department of the Rafael Rangel National Institute of Hygiene, was made. Secondary alcohol-dehydrogenase gene amplification was performed using polymerase chain reaction, and the products were analysed by restriction fragments length polymorphisms using the enzyme BanI. Susceptibility tests were performed using Etest (R), following the manufacturer's instructions with modifications.Results: Of the 86 isolates studied, 81 (94.2%) were C. parapsilosis sensu stricto, 4 (4.6%) C. orthopsilosis, and one (1.2%) C. metapsilosis. C. parapsilosis isolates were susceptible to amphotericin B and caspofungin, showing low rates of resistance to fluconazole and voriconazole. C. orthopsilosis and C. metapsilosis were susceptible to all the antifungals tested.Conclusions: The results obtained in Venezuela provide for the first time important information about the distribution of C. parapsilosis species complex in cases of candidaemia, and support the need for continuing surveillance programs, including molecular discrimination of species and antifungal susceptibility tests, which may guide specific therapy. (C) 2017 Asociacion Espanola de Micologia. Published by Elsevier Espana, S.L.U. All rights reserved.