Coordinated and Distinct Functions of Velvet Proteins in Fusarium verticillioides
Coordinated and Distinct Functions of Velvet Proteins in Fusarium verticillioides
复制标题
轮枝镰刀菌绒蛋白的协调和独特功能
DOI：
10.1128/ec.00022-14
复制
发表时间：
2014-07-01
期刊：
影响因子：
--
通讯作者：
Li, Shaojie
中科院分区：
文献类型：
--
作者：
Lan, Nan;Zhang, Hanxing;Li, Shaojie
摘要天鹅绒结构域蛋白广泛分布于真菌界。在玉米病原菌轮枝镰刀菌（Fusarium verticillioides）中，以往的研究表明，鹿茸蛋白F.轮枝菌VE1（FvVE1）对形态发育、菌落疏水性、毒素产生和致病性至关重要。在本研究中，FvVE 1的串联亲和纯化表明，FvVE 1可以与天鹅绒蛋白F形成复合物。verticillioides VelB（FvVelB）和FvVelC.基因敲除突变体的表型表征表明，在FvVE1的情况下，FvVelB调节分生孢子的大小，菌丝疏水性，伏马菌素的生产，和抗氧化性，而FvVelC是这些生物过程的抑制剂。对参与活性氧清除系统的8个基因的比较转录分析表明，FvVE1和FvVelB均正向调节过氧化氢酶编码基因F.轮枝菌CAT 2（FvCAT 2）。FvCAT2的缺失导致抗氧化性降低，进一步解释了真菌界中天鹅绒蛋白对抗氧化性的调节。
ABSTRACT Velvet-domain-containing proteins are broadly distributed within the fungal kingdom. In the corn pathogen Fusarium verticillioides, previous studies showed that the velvet protein F. verticillioides VE1 (FvVE1) is critical for morphological development, colony hydrophobicity, toxin production, and pathogenicity. In this study, tandem affinity purification of FvVE1 revealed that FvVE1 can form a complex with the velvet proteins F. verticillioides VelB (FvVelB) and FvVelC. Phenotypic characterization of gene knockout mutants showed that, as in the case of FvVE1, FvVelB regulated conidial size, hyphal hydrophobicity, fumonisin production, and oxidant resistance, while FvVelC was dispensable for these biological processes. Comparative transcriptional analysis of eight genes involved in the ROS (reactive oxygen species) removal system revealed that both FvVE1 and FvVelB positively regulated the transcription of a catalase-encoding gene, F. verticillioides CAT2 (FvCAT2). Deletion of FvCAT2 resulted in reduced oxidant resistance, providing further explanation of the regulation of oxidant resistance by velvet proteins in the fungal kingdom.