Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects.
Messenger RNA expression of the cytokine gene cluster, interleukin 3 (IL-3), IL-4, IL-5, and granulocyte/macrophage colony-stimulating factor, in allergen-induced late-phase cutaneous reactions in atopic subjects.
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在过敏原诱导的晚期相期皮肤反应中，细胞因子基因簇，白介素3（IL-3），IL-3），IL-4，IL-5和粒细胞/巨噬细胞刺激因子的信使RNA表达。
DOI：
10.1084/jem.173.3.775
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发表时间：
1991-03-01
期刊：
影响因子：
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通讯作者：
Hamid Q
中科院分区：
文献类型：
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作者：
Kay AB;Ying S;Varney V;Gaga M;Durham SR;Moqbel R;Wardlaw AJ;Hamid Q
从14名人类特应性受试者的24小时变应原诱导的晚期皮肤反应(LPR)的皮肤活检中提取的冰冻切片与35S标记的RNA探针杂交，以检测一些细胞因子。IL-3(8/14)、IL-4(10/14)、IL-5(11/14)、粒细胞/巨噬细胞集落刺激因子(GM-CSF)(13/14)表达。14例中只有5例出现IL-2杂交信号，0例出现干扰素-γ杂交信号。稀释剂对照组的活组织检查只给出了偶尔的微弱信号。这些结果表明，渗透到24小时LPR转录位点的细胞可以转录IL-3、IL-4、IL-5和GM-CSF基因簇的mRNA，并支持特应性与细胞因子谱与小鼠辅助性T细胞2型亚群相似的细胞的优先激活有关的假设。
Cryostat sections from skin biopsies from 24-h allergen-induced late- phase cutaneous reactions (LPR) in 14 human atopic subjects were hybridized with 35S-labeled RNA probes for a number of cytokines. mRNA was detected for interleukin 3 (IL-3) (8/14), IL-4 (10/14), IL-5 (11/14), and granulocyte/macrophage colony-stimulating factor (GM-CSF) (13/14). Only 5 of 14 gave hybridization signals for IL-2, and 0 of 14 for interferon gamma. Biopsies from diluent controls gave only occasional weak signals. These results suggest that cells infiltrating the site of the 24-h LPR transcribe mRNA for the IL-3, IL-4, IL-5, and GM-CSF gene cluster and support the hypothesis that atopy is associated with preferential activation of cells having a similar cytokine profile to the murine T helper type 2 subset.