Expression of Semaphorins, Neuropilins, VEGF, and Tenascins in Rat and Human Primary Sensory Neurons after a Dorsal Root Injury.
Expression of Semaphorins, Neuropilins, VEGF, and Tenascins in Rat and Human Primary Sensory Neurons after a Dorsal Root Injury.
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DOI：
10.3389/fneur.2017.00049
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发表时间：
2017
影响因子：
3.4
通讯作者：
Sköld MK
中科院分区：
文献类型：
--
作者：
Lindholm T;Risling M;Carlstedt T;Hammarberg H;Wallquist W;Cullheim S;Sköld MK
背根损伤是一种情况，如果背根的中央轴突受损，则预期不会发生强再生生长，或受损轴突生长到脊髓的中枢神经系统中，但如果受到外周突出轴突的损伤，则预期不会发生强再生。轴突损伤的临床后果是感觉丧失，也可能导致神经性疼痛。在这项研究中，我们使用原位杂交来检测神经导向分子脑信号蛋白3A（SEMA 3A），脑信号蛋白3F（SEMA 3F）和脑信号蛋白4F（SEMA 4F），它们的受体神经纤毛蛋白1（NP 1）和神经纤毛蛋白2（NP 2）以及神经纤毛蛋白配体血管内皮生长因子（VEGF）和腱生蛋白J1的mRNA分布，一种参与轴突导向的细胞外基质分子，在单侧背根切断术（DRT）或坐骨神经移植术（SNT）后的大鼠背根神经节（DRG）中。研究的存活时间为1-365天。不同形式的mRNA在不同大小的感觉神经细胞之间分布不均匀。结果显示，大鼠感觉神经根创伤后SEMA 3A的mRNA减少。SEMA 3A受体NP 1和SEMA 3F受体NP 2在DRT和SNT后在DRG神经元中显著上调。SEMA 4F在SNT后上调。DRT后DRG神经元VEGF mRNA的表达显著上调，即使在损伤后一年也很高。这些数据表明，脑信号蛋白，神经纤毛蛋白，血管内皮生长因子和J1在背根损伤后的反应中的作用。
Dorsal root injury is a situation not expected to be followed by a strong regenerative growth, or growth of the injured axon into the central nervous system of the spinal cord, if the central axon of the dorsal root is injured but of strong regeneration if subjected to injury to the peripherally projecting axons. The clinical consequence of axonal injury is loss of sensation and may also lead to neuropathic pain. In this study, we have used in situ hybridization to examine the distribution of mRNAs for the neural guidance molecules semaphorin 3A (SEMA3A), semaphorin 3F (SEMA3F), and semaphorin 4F (SEMA4F), their receptors neuropilin 1 (NP1) and neuropilin 2 (NP2) but also for the neuropilin ligand vascular endothelial growth factor (VEGF) and Tenascin J1, an extracellular matrix molecule involved in axonal guidance, in rat dorsal root ganglia (DRG) after a unilateral dorsal rhizotomy (DRT) or sciatic nerve transcetion (SNT). The studied survival times were 1–365 days. The different forms of mRNAs were unevenly distributed between the different size classes of sensory nerve cells. The results show that mRNA for SEMA3A was diminished after trauma to the sensory nerve roots in rats. The SEMA3A receptor NP1, and SEMA3F receptor NP2, was significantly upregulated in the DRG neurons after DRT and SNT. SEMA4F was upregulated after a SNT. The expression of mRNA for VEGF in DRG neurons after DRT showed a significant upregulation that was high even a year after the injuries. These data suggest a role for the semaphorins, neuropilins, VEGF, and J1 in the reactions after dorsal root lesions.