TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization
TopPIC: a software tool for top-down mass spectrometry-based proteoform identification and characterization
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DOI：
10.1093/bioinformatics/btw398
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发表时间：
2016-11-15
期刊：
影响因子：
5.8
通讯作者：
Liu, Xiaowen
中科院分区：
文献类型：
--
作者：
Kou, Qiang;Xun, Likun;Liu, Xiaowen
自上而下质谱法能够观察生物样品中的整个复杂蛋白质组，并提供与自下而上质谱法互补的关键信息。由于自上而下质谱和蛋白质组的复杂性，在高通量蛋白质组水平的蛋白质组学研究中，有效地解释自上而下串联质谱是一个具有挑战性的问题。我们提出TopPIC，一种工具，有效地识别和表征复杂的蛋白质与未知的一级结构改变，如氨基酸突变和翻译后修饰，通过搜索自上而下的串联质谱对蛋白质数据库。
Top-down mass spectrometry enables the observation of whole complex proteoforms in biological samples and provides crucial information complementary to bottom-up mass spectrometry. Because of the complexity of top-down mass spectra and proteoforms, it is a challenging problem to efficiently interpret top-down tandem mass spectra in high-throughput proteome-level proteomics studies. We present TopPIC, a tool that efficiently identifies and characterizes complex proteoforms with unknown primary structure alterations, such as amino acid mutations and post-translational modifications, by searching top-down tandem mass spectra against a protein database.