Influence of glycogen storage on vascular smooth muscle metabolism.
Influence of glycogen storage on vascular smooth muscle metabolism.
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糖原储存对血管平滑肌代谢的影响。
DOI：
10.1152/ajpheart.2000.278.6.h1993
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Hardin,CD
中科院分区：
文献类型：
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作者：
Allen,TJ;Hardin,CD
糖原作为血管平滑肌（VSM）的氧化底物的作用仍然存在争议。为了阐明糖原作为氧化底物的重要性和糖原通量对VSM底物选择的影响，我们系统地改变了糖原水平并测量了葡萄糖、乙酸盐和糖原的代谢。糖原含量为1 ~ 11 μmol/g的猪颈动脉在含5 mM [1- 13 C]葡萄糖和1 mM [1，2 - 13 C]乙酸盐的生理盐溶液中于37°C等容收缩6 h。[1- 13 C]葡萄糖，[1，2 - 13 C]乙酸，和糖原氧化的同时测量与使用α 13 C-标记的同位素分析谷氨酸。虽然糖原的氧化随着组织中糖原含量的增加而增加，但糖原氧化仅占VSM氧化底物的约10%。而[1- 13 C]葡萄糖通量、[1- 13 C]葡萄糖产生的[3- 13 C]乳酸和[1，2 - 13 C]乙酸氧化不受糖原含量的调节，[1- 13 C]葡萄糖氧化受VSM糖原含量的显著影响。然而，[1- 13 C]葡萄糖仍然是底物氧化的主要贡献者（约40-50%）。因此，我们得出结论，葡萄糖是主要的底物氧化的VSM，和糖原的氧化贡献最小的底物氧化。
The role of glycogen as an oxidative substrate for vascular smooth muscle (VSM) remains controversial. To elucidate the importance of glycogen as an oxidative substrate and the influence of glycogen flux on VSM substrate selection, we systematically altered glycogen levels and measured metabolism of glucose, acetate, and glycogen. Hog carotid arteries with glycogen contents ranging from 1 to 11 μmol/g were isometrically contracted in physiological salt solution containing 5 mM [1-13C]glucose and 1 mM [1,2-13C]acetate at 37°C for 6 h. [1-13C]glucose, [1,2-13C]acetate, and glycogen oxidation were simultaneously measured with the use of a13C-labeled isotopomer analysis of glutamate. Although oxidation of glycogen increased with the glycogen content of the tissue, glycogen oxidation contributed only ∼10% of the substrate oxidized by VSM. Whereas [1-13C]glucose flux, [3-13C]lactate production from [1-13C]glucose, and [1,2-13C]acetate oxidation were not regulated by glycogen content, [1-13C]glucose oxidation was significantly affected by the glycogen content of VSM. However, [1-13C]glucose remained the primary (∼40–50%) contributor to substrate oxidation. Therefore, we conclude that glucose is the predominate substrate oxidized by VSM, and glycogen oxidation contributes minimally to substrate oxidation.