In vivo effect of volume expansion on rectal gland function. I. Humoral factors.
In vivo effect of volume expansion on rectal gland function. I. Humoral factors.
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体内体积扩张对直肠腺功能的影响。
DOI：
10.1152/ajpregu.1984.246.1.r63
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发表时间：
1984
期刊：
影响因子：
--
通讯作者：
Epstein,FH
中科院分区：
文献类型：
--
作者：
Solomon,R;Taylor,M;Stoff,JS;Silva,P;Epstein,FH
角鲨通过增加其直肠腺的氯化物分泌率来应对体积膨胀。该反应是通过血管内输注等渗鲨鱼林格溶液、1 M 高渗氯化钠溶液或含有等体积鲨鱼林格溶液和 10% 甘露醇的等渗低钠溶液引起的。仅通过动脉供应在与宿主鱼连接的移植腺体中引起体积膨胀的效应，表明该反应是由体液因子介导的。移植的腺体对茶碱（2.5 X 10(-3) M）和腺苷3',5'-环单磷酸（5 X 10(-4) M）作出反应，增加氯化物的分泌速率，其量与灌注鱼体积膨胀引起的量相似。已知浓度（10(-6) 至 5 X 10(-5) M）的茶碱可抑制离体灌注腺体中腺苷的作用，但无法抑制外植腺体体积扩张的作用。生长抑素（4.5 X 10(-6) M）可抑制血管活性肠肽（VIP）在分离的灌注腺体中的作用，完全阻止外植腺体对体积扩张的分泌反应。体积膨胀是直肠腺分泌氯离子的主要刺激因素。该效应是由一种体液因子介导的，该因子似乎是 VIP。
The spiny dogfish Squalus acanthias responds to volume expansion by increasing the rate of chloride secretion by its rectal gland. The response is elicited by intravascular infusion of either isotonic shark Ringer solution, a 1 M hypertonic sodium chloride solution, or an isotonic hyponatremic solution containing equal volumes of shark Ringer solution and 10% mannitol. The effect of volume expansion was evoked in explanted glands connected to a host fish only by the arterial supply, indicating that the response is mediated by a humoral factor. The explanted gland responded to theophylline (2.5 X 10(-3) M) and adenosine 3',5'-cyclic monophosphate (5 X 10(-4) M) by increasing the rate of secretion of chloride by an amount similar to that induced by volume expansion of the perfusing fish. Theophylline at concentrations (10(-6) to 5 X 10(-5) M) that are known to inhibit the effect of adenosine in isolated perfused glands failed to inhibit the effect of volume expansion on explanted glands. Somatostatin (4.5 X 10(-6) M), which inhibits the effect of vasoactive intestinal peptide (VIP) in the isolated perfused gland, completely prevented the secretory response to volume expansion in explanted glands. Volume expansion is a major stimulus for chloride secretion by the rectal gland. The effect is mediated by a humoral factor that appears to be VIP.