IFN-γ Production from Liver Mononuclear Cells of Mice in Burn Injury As Well As in Postburn Bacterial Infection Models and the Therapeutic Effect of IL-18
IFN-γ Production from Liver Mononuclear Cells of Mice in Burn Injury As Well As in Postburn Bacterial Infection Models and the Therapeutic Effect of IL-18
复制标题
烧伤及烧伤后细菌感染模型中小鼠肝单核细胞产生的 IFN-γ 及 IL-18 的治疗作用
作者：
Katsunori Ami;M. Kinoshita;A. Yamauchi;T. Nishikage;Y. Habu;N. Shinomiya;T. Iwai;H. Hiraide;S. Seki
严重烧伤后的宿主已知在Th1免疫反应中存在缺陷，容易受到细菌感染。我们在这里表明，烧伤后早期肝脏NK细胞是产生干扰素-γ的有效细胞。然而，烧伤后24 h注射脂多糖后，小鼠肝脏单个核细胞产生的干扰素-γ受到抑制，血清干扰素-γ浓度没有升高，而血清IL-10浓度则相反。有趣的是，一次性注射IL-18同时注射脂多糖可显著恢复烧伤小鼠血清中干扰素-γ的浓度，并增加肝单个核细胞产生干扰素-γ的量。然而，一次性注射IL-18并同时注射脂多糖不能恢复烧伤后7天小鼠血清干扰素-γ和肝单个核细胞干扰素-γ的产生，此时小鼠被认为是免疫功能最差的。而从烧伤后第1天开始隔日注射IL-18可显著上调内毒素诱导的小鼠伤后7天血清干扰素-γ水平和肝、脾单个核细胞产生干扰素-γ，下调血清IL-10。此外，类似的IL-18治疗可上调烧伤后7天实验性细菌性腹膜炎小鼠的血清干扰素-γ水平，并显著降低小鼠死亡率。因此，IL-18治疗可恢复烧伤小鼠的Th1反应，并可能降低其对细菌感染的易感性。
Hosts after severe burn injury are known to have a defect in the Th1 immune response and are susceptible to bacterial infections. We herein show that liver NK cells are potent IFN-γ producers early after burn injury. However, when mice were injected with LPS 24 h after burn injury, IFN-γ production from liver mononuclear cells (MNC; which we previously showed to be NK cells) was suppressed, and the serum IFN-γ concentration did not increase, while serum IL-10 conversely increased compared with control mice. Interestingly, a single injection of IL-18 simultaneously with LPS greatly restored the serum IFN-γ concentration in mice with burn injury and also increased IFN-γ production from liver MNC. Nevertheless, a single IL-18 injection into mice simultaneously with LPS was no longer effective in the restoration of serum IFN-γ and IFN-γ production from the liver MNC at 7 days after burn injury, when mice were considered to be the most immunocompromised. However, IL-18 injections into mice on alternate days beginning 1 day after burn injury strongly up-regulated LPS-induced serum IFN-γ levels and IFN-γ production from liver and spleen MNC of mice 7 days after burn injury and down-regulated serum IL-10. Furthermore, similar IL-18 therapy up-regulated serum IFN-γ levels in mice with experimental bacterial peritonitis 7 days after burn injury and greatly decreased mouse mortality. Thus, IL-18 therapy restores the Th1 response and may decrease the susceptibility to bacterial infection in mice with burn injury.
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影响因子：
9
作者：
Göebel, A;Kavanagh, E;Rodrick, ML
通讯作者：
Rodrick, ML
DOI：
10.1016/s0021-9258(17)37218-6
发表时间：
1994-03
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Baiba J. Grube;Charles G. Cochane;Richard D. Ye;Carol E. Green;Mary E. McPhail;R. Ulevitch;Peter S. Tobias
通讯作者：
Baiba J. Grube;Charles G. Cochane;Richard D. Ye;Carol E. Green;Mary E. McPhail;R. Ulevitch;Peter S. Tobias
Interleukin-12 治疗可恢复烧伤后对细菌攻击的正常抵抗力。
影响因子：
3.8
作者：
O'Suilleabhain,C;O'Sullivan,ST;Kelly,JL;Lederer,J;Mannick,JA;Rodrick,ML
通讯作者：
Rodrick,ML
影响因子：
3.8
作者：
Kelly, JL;Lyons, A;Lederer, JA
通讯作者：
Lederer, JA