MicroRNA Expressions in PMBCs, CD4+, and CD8+T-Cells from Patients Suffering from Autoimmune Addison's Disease
MicroRNA Expressions in PMBCs, CD4+, and CD8+T-Cells from Patients Suffering from Autoimmune Addison's Disease
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DOI：
10.1055/s-0033-1341511
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发表时间：
2013-08-01
影响因子：
2.2
通讯作者：
Schott, M.
中科院分区：
文献类型：
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作者：
Bernecker, C.;Halim, F.;Schott, M.
自身免疫性阿狄森氏病（AD）是一种罕见但可能危及生命的疾病。肾上腺免疫反应的确切病因仍然未知。microRNA（miRNAs）是基因表达的关键调控因子，在免疫应答中起重要作用。本研究的目的是确定影响自身免疫性肾上腺皮质功能不全的关键免疫调节miRNAs。为此，通过半定量SYBR绿色PCR从6名自身免疫性肾上腺功能不全患者和10名健康对照的血液单核细胞中以及从CD 4+和CD 8+细胞纯化后扩增选定的miRNA。在CD4+ T细胞中，miRNA 181a*_1（AD中18.02对CG中11.99，p = 0.0047）显著增加，而miRNA 200a_1（AD中12.48对比CG中19.40，p = 0.0003）和miRNA 200a_2*（AD中8.59对比CG中17.94，p = 0.0160）显著降低。miRNA 200a_1（AD组12.37对对照组18.12，p = 0.001）和miRNA 200a_2*（AD组10.72对对照组17.84，p = 0.022）在CD8+ T细胞中也显著降低。该研究首次显示了自身免疫性AD患者体内PBMC、CD4+和CD8+ T细胞中三种定义的miRNA的显著变化。这些数据可能有助于更好地了解导致自身免疫性AD的自身免疫过程的原因。他们扩展了我们关于自身免疫性阿狄森病中miRNAs的非常有限的知识。
Autoimmune Addison's disease (AD) is a rare but potentially life threatening disease. The exact etiology of the immune response to the adrenal gland is still unknown. MicroRNAs (miRNAs) critically control gene-expression and play an important role in regulating the immune response. The aim of this study was to determine key immunoregulatory miRNAs influencing autoimmune adrenal insufficiency. For this purpose selected miRNAs were amplified by a semiquantitative SYBR Green PCR from blood mononuclear cells and after purification from CD4+ and CD8+ cells of 6 patients with autoimmune adrenal insufficiency and 10 healthy controls. In CD4+ T-cells miRNA 181a*_1 (18.02 in AD vs. 11.99 in CG, p = 0.0047) is significantly increased whereas miRNA 200a_1 (12.48 in AD vs. 19.40 in CG, p = 0.0003) and miRNA 200a_2* (8.59 in AD vs. 17.94 in CG, p = 0.0160) are significantly decreased. miRNA 200a_1 (12.37 in AD group vs. 18.12 in control group, p = 0.001) and miRNA 200a_2* (10.72 in AD group vs. 17.84 in control group, p = 0.022) are also significantly decreased in CD8+ T-cells. This study could show for the first time a significant change of three defined miRNAs in PBMCs, CD4+, and CD8+ T-cells of autoimmune AD patients in vivo. These data may help to better understand the cause of the autoimmune processes leading to autoimmune AD. They extend our very limited knowledge concerning miRNAs in autoimmune Addison's disease.