Preferential translation of internal ribosome entry site-containing mRNAs during the mitotic cycle in mammalian cells
Preferential translation of internal ribosome entry site-containing mRNAs during the mitotic cycle in mammalian cells
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DOI：
10.1074/jbc.m312854200
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发表时间：
2004-04-02
影响因子：
4.8
通讯作者：
Sarnow, P
中科院分区：
文献类型：
--
作者：
Qin, XL;Sarnow, P
建立了一种细胞同步化方案，其中可以检查全局和个体mRNA的翻译效率。虽然在有丝分裂细胞中总体翻译效率降低，但通过cDNA微阵列分析确定，在有丝分裂期间，约3%的mRNA仍然主要与大的多聚核糖体相关。6个mRNA的5 '非编码区均含有内部核糖体进入位点（IRES）。然而，并不是所有已知的含有IRES元件的mRNA在有丝分裂过程中都被积极翻译，这表明特定的IRES序列在有丝分裂过程中受到差异调节。
A cell synchronization protocol was established in which global and individual mRNA translational efficiencies could be examined. While global translational efficiency was reduced in mitotic cells, similar to 3% of mRNAs remained predominantly associated with large polysomes during mitosis, as determined by cDNA microarray analyses. The 5'-non-coding regions of six mRNAs were shown to contain internal ribosome entry sites (IRES). However, not all known mRNAs that contain IRES elements were actively translated during mitosis, arguing that specific IRES sequences are differentially regulated during mitosis.