Molecular characterization and detection of a tripartite cryptic virus from rose.
Molecular characterization and detection of a tripartite cryptic virus from rose.
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DOI：
10.4454/jpp.v90i2.664
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发表时间：
2008-07
影响因子：
2.2
通讯作者：
S. Sabanadzovic;N. A. Ghanem-Sabanadzovic
中科院分区：
文献类型：
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作者：
S. Sabanadzovic;N. A. Ghanem-Sabanadzovic
摘要从无症状杂交茶树月季品种中提取了三个dsRNA分子，其分子量分别为1.7、1.5和1.4kBP。纯粹的极乐，并进一步刻画。对全序列的分析表明，它们代表了一种三方隐蔽病毒的基因组片段，建议将其命名为玫瑰隐蔽病毒1(RCV-1)。这三个片段都是单顺反子，dsRNA-1编码的蛋白质含有依赖RNA的RNA聚合酶的保守基序。DsRNA2和dsRNA3编码的两种蛋白质有大约20%的共同氨基酸，可能参与了病毒衣壳的组成。基于病毒RdRp基因的系统发育分析表明，RCV-1与新近发现的三部分隐蔽病毒FCCV和RsCV-2的亲缘关系最近。有趣的是，这组病毒似乎在系统发育上更接近于属于部分病毒属的分支病毒，而不是感染植物的隐形病毒。RT-PCR在不同品种/杂交种的供试样品中检测到约30%的病毒，表明该病毒可能在月季种质中相当普遍。
SUMMARY Three dsRNA molecules with estimated molecular weights of c. 1.7, 1.5 and 1.4 kbp were extracted from a symptomless hybrid tea rose cv. Sheer Bliss, and further characterized. Analysis of complete sequences showed that they represent genome segments of a tripartite cryptic virus with the proposed name Rose cryptic virus 1 (RCV-1). All three segments were monocistronic, with the dsRNA-1 encoding a protein containing conserved motifs of RNA-dependent RNA polymerases. The two proteins encoded by dsRNA 2 and 3 shared c. 20% common amino acids and are probably involved in the make up of the viral capsid. Phylogenetic analysis based on the viral RdRp gene showed closest relationships of RCV-1 with Fragaria chiloensis cryptic virus (FCCV) and Raphanus sativus cryptic virus 2 (RsCV-2), both recently described tripartite cryptoviruses. Interestingly, this group of viruses appears to be phylogenetically closer to mycoviruses belonging in the genus Partitivirus than to plant-infecting cryptoviruses. The virus was detected by RT-PCR in ca. 30% of the tested samples belonging to different cultivars/hybrids, indicating that it may be quite common in rose germplasm.