A hybrid quantum dot-antibody fragment fluorescence resonance energy transfer-based TNT sensor
A hybrid quantum dot-antibody fragment fluorescence resonance energy transfer-based TNT sensor
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DOI：
10.1021/ja043677l
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发表时间：
2005-05-11
影响因子：
15
通讯作者：
Mattoussi, H
中科院分区：
文献类型：
--
作者：
Goldman, ER;Medintz, IL;Mattoussi, H
我们展示了利用结合抗体片段的发光量子点来开发基于荧光共振能量转移（FRET）的液相纳米级传感组件，用于在水环境中特异性检测爆炸性2,4,6-三硝基甲苯（TNT）。该混合传感器由抗tnt特异性抗体片段通过金属亲和配位连接到亲水性量子点组成。染料标记的TNT类似物预结合在抗体结合位点，通过邻近诱导的FRET猝灭QD光致发光。通过增加染料标记的类似物与QD的比例来分析收集的数据，可以深入了解抗体片段如何在QD上自组装。添加可溶性TNT取代了染料标记的类似物，消除了FRET，导致量子点光致发光的浓度依赖恢复。评估传感器的性能和特异性。
We demonstrate the use of luminescent QDs conjugated to antibody fragments to develop solution-phase nanoscale sensing assemblies, based on fluorescence resonance energy transfer (FRET) for the specific detection of the explosive 2,4,6-trinitrotoluene (TNT) in aqueous environments. The hybrid sensor consists of anti-TNT specific antibody fragments attached to a hydrophilic QD via metal-affinity coordination. A dye-labeled TNT analogue prebound in the antibody binding site quenches the QD photoluminescence via proximity-induced FRET. Analysis of the data collected at increasing dye-labeled analogue to QD ratios provided an insight into understanding how the antibody fragments self-assemble on the QD. Addition of soluble TNT displaces the dye-labeled analogue, eliminating FRET and resulting in a concentration-dependent recovery of QD photoluminescence. Sensor performance and specificity were evaluated.