CYTOSKELETAL COMPONENTS OF THE VERTEBRATE NEUROMUSCULAR-JUNCTION - VINCULIN, ALPHA-ACTININ, AND FILAMIN
CYTOSKELETAL COMPONENTS OF THE VERTEBRATE NEUROMUSCULAR-JUNCTION - VINCULIN, ALPHA-ACTININ, AND FILAMIN
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DOI：
10.1083/jcb.97.1.217
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发表时间：
1983-01-01
影响因子：
7.8
通讯作者：
HALL, ZW
中科院分区：
文献类型：
--
作者：
BLOCH, RJ;HALL, ZW
免疫细胞化学方法用于研究脊椎动物神经肌肉接头的细胞骨架成分。针对 3 种细胞骨架蛋白（纽蛋白、α-辅肌动蛋白和纤丝蛋白）的特异性亲和纯化抗体，与正常大鼠、小鼠、小鸡和非洲爪蟾肌肉切片中的神经肌肉接头结合。所有 3 种抗体均与去神经大鼠肌纤维的突触区域结合，表明这些抗体识别的蛋白质与突触后结构相关。这 3 种蛋白质存在于胚胎和新生动物肌纤维的神经肌肉接头处，可能在其分化中发挥重要作用。
Immunocytochemical methods were used to investigate the cytoskeletal constituents of the vertebrate neuromuscular junction. Specific, affinity-purified antibodies to 3 cytoskeletal proteins, vinculin, .alpha.-actinin and filamin, bound to neuromuscular junctions in sections of normal rat, mouse, chick and Xenopus muscles. All 3 antibodies bound to the synaptic regions of denervated rat muscle fibers, indicating that the proteins recognized by these antibodies are associated with postsynaptic structures. The 3 proteins are present at the neuromuscular junction in muscle fibers of embryonic and neonatal animals and may play an important role in its differentiation.