Intestinal Anti-inflammatory Activity of Dietary Fiber (plantago Ovata Seeds) in Hla-b27 Transgenic Rats
Intestinal Anti-inflammatory Activity of Dietary Fiber (plantago Ovata Seeds) in Hla-b27 Transgenic Rats
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通讯作者：
M. E. Rodrił Guez-Cabezas;J. Ga Ł Lvez;D. Camuesco;M. D. Lorente;Á. Concha;O. Martínez-Augustin;L. Redondo;A. Zarzuelo
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作者：
M. E. Rodrił Guez-Cabezas;J. Ga Ł Lvez;D. Camuesco;M. D. Lorente;Á. Concha;O. Martínez-Augustin;L. Redondo;A. Zarzuelo
研究背景与目的：事实证明，饮食中的维生素B对维持人类溃疡性结肠炎的缓解是有益的，这与短链脂肪酸(SCFA)的合成增加有关。本研究的目的是在实验性大鼠结肠炎模型上进一步探讨膳食Ⅱ的肠道抗炎作用机制。方法：给8~10周龄的转基因大鼠喂饲含5%车前子的饲料13周后，从组织学和生化角度评价结肠炎性状态。定量测定SCFA的肠腔分泌量。体外实验还检测了丁酸和丙酸两种单链脂肪酸对THP-1细胞产生细胞因子的抑制作用。结果：饮食补充能改善转基因大鼠结肠炎的发育，主要表现为肠道细胞结构的改善，这与参与肠炎化过程的某些促炎性介质：一氧化氮、白三烯B4、肿瘤坏死因子a的减少有关。治疗组大鼠肠内容物中丁酸和丙酸的生成量显著高于未治疗组。体外研究表明，丁酸和丙酸对肿瘤坏死因子α的产生有协同抑制作用。结论：饮食补充能改善HLAB27转基因大鼠的结肠损伤，这与SCFA的产生增加有关，而SCFA在抑制Gb前炎症介质的产生方面具有协同作用。
ö Background & aims: Dietary ¢ber has been proven to be bene¢cial in maintaining remission in human ulcera-tive colitis, an e¡ect related with an increased luminal production of short-chain fatty acids (SCFA). The aim of the present study was to further investigate the mechanisms involved in the intestinal anti-in£ammatory e¡ects of dietary ¢ber in an experimental model of rat colitis. Methods: HLA-B27 transgenic rats (8^10 weeks old) were fed a ¢ber-supplemented diet (5% Plantago ovata seeds) for 13 weeks before evaluation of the colonic in£ammatory status, both histologically and biochemically. The luminal colonic production of SCFA was quanti¢ed. In vitro studies were also performed to test the interaction between two SCFA (butyrate and propionate) as inhibitors of cytokine production inTHP-1cells. Results: Dietary ¢ber supplementation ameliorated the development of colonic in£ammation in transgenic rats as evi-denced by an improvement of intestinal cytoarchitecture.This e¡ect was associated with a decrease in some of the pro-in£ammatory mediators involvedin the in£ammatory process: nitric oxide, leukotriene B 4 , tumor necrosis factor a (TNFa). The intestinal contents from ¢ber-treated colitic rats showed a signi¢cant higher production of SCFA, butyrate and pro-pionate, than non-treated colitic animals. In vitro studies revealed a synergistic inhibitory e¡ect of butyrate and propio-nate onTNFa production. Conclusions: Dietary ¢ber supplementation ameliorated colonic damage in HLA-B27 transgenic rats.This e¡ects was associated with an increased production of SCFA, which can act synergistically in inhibiting the production of pro-in£ammatory mediators.