Atrial Natriuretic Factor: Characterization and Partial Purification
Atrial Natriuretic Factor: Characterization and Partial Purification
复制标题
心房钠尿因子：表征和部分纯化
DOI：
--
复制
发表时间：
1983
期刊：
影响因子：
--
通讯作者：
A. Genest
中科院分区：
文献类型：
--
作者：
G. Thibault;Raúl García;M. Cantin;A. Genest
心房和心室心肌细胞之间的主要差异之一是前者存在具有与肽分泌内分泌细胞中发现的分泌颗粒非常相似的形态特征的特定颗粒。有人认为这些颗粒是心房利钠因子的储存场所。在大鼠中，水剥夺产生心房颗粒的增加，但酸可提取的利尿剂和利钠活性显着降低，这表明心房特定颗粒的数量不一定代表利钠活性。心房利钠因子的活性被几种蛋白酶破坏，并且不抑制钠-钾ATP酶，这表明活性物质是一种小肽，可能与所谓的利钠激素不同。在Sep-Pak柱、Bio-Gel P-IO、CM Bio-Gel和Mono S柱中进行一系列色谱步骤后，心房利钠因子的比活性从193（对应于心房匀浆）增加到242，000（对应于最后一个色谱步骤，代表1250倍纯化）。该物质显示出有效的利钠活性，因为10皮摩尔使利钠作用增加100°。
One of the main differences between atrial and ventricular cardiocytes is the presence in former of specific granules with morphological characteristics very similar to secretory granules found in peptide-secreting endocrine cells. It has been suggested that these granules are the storage place for the atrial natriuretic factor. In the rat, water deprivation produces an increase in atrial granularity but a significant decrease in acid-extractable diuretic and natriuretic activity, suggesting that the number of atrial specific granules does not necessarily represent natriuretic activity. The atrial natriuretic factor activity is destroyed by incubation with several proteases and does not inhibit the sodium-potassium ATPase, suggesting that the active substance is a small peptide that is probably different from the so-called natriuretic hormone. After a series of chromatographic steps in Sep-Pak cartridges, Bio-Gel P-IO, CM Bio-Gel, and Mono S columns, the specific activity of the atrial natriuretic factor was increased from 193, corresponding to atrial homogenates, to 242,000, which corresponds to the last chromatographic step representing a 1250-fold purification. This material showed a potent natriuretic activity, as 10 picomoles increased natriuresis by 100°.