Modulation of VEGF expression in rat bone marrow stromal cells by GDF-5
Modulation of VEGF expression in rat bone marrow stromal cells by GDF-5
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DOI：
10.1080/03008200701692743
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发表时间：
2007-01-01
影响因子：
2.9
通讯作者：
Virdi, Amarjit S.
中科院分区：
文献类型：
--
作者：
Sena, Kotaro;Sumner, Dale R.;Virdi, Amarjit S.
血管生成是骨形成所必需的，并且已经显示几种骨形态发生蛋白（BMP）通过成骨细胞衍生的血管内皮生长因子（VEGF）-A诱导血管生成。生长分化因子-5（GDF-5）是骨中表达的BMP家族成员，已知可诱导体内血管生成。在这项研究中，GDF-5对成骨分化和VEGF相关基因的表达的影响，确定使用大鼠骨髓基质细胞。GDF-5刺激成骨分化。VEGF-D及其受体VEGFR-3表达高峰出现在较晚的时间点。这种调节可能进一步控制神经纤毛蛋白2，表现出一个平行的轮廓VEGF-D。这些观察结果表明，GDF-5刺激成骨分化，并具有通过骨中成骨细胞衍生的VEGF-A诱导血管生成的潜力。
Angiogenesis is essential for bone formation and several bone morphogenetic proteins (BMPs) have been shown to induce angiogenesis through osteoblast-derived vascular endothelial growth factor (VEGF)-A. Growth differentiation factor-5 (GDF-5) is a member of the BMP family expressed in bone and known to induce angiogenesis in vivo. In this study, the effects of GDF-5 on osteogenic differentiation and expression of VEGF-related genes were determined using rat bone marrow stromal cells. GDF-5 stimulated osteogenic differentiation. It also upregulated the expression of VEGF-A after 3 hr, accompanied by a trend of decrease in its receptor VEGFR-2 at 6 and 24 hr. VEGF-D and its receptor VEGFR-3 showed peak expression at later time points. This regulation may be further controlled by neuropilin 2 that exhibited a parallel profile to VEGF-D. These observations indicate that GDF-5 stimulates osteogenic differentiation and has a potential to induce angiogenesis through osteoblast-derived VEGF-A in bone.