Molecular Epidemiological Survey of the Babesia gibsoni cytochrome b Gene in Western Japan
Molecular Epidemiological Survey of the Babesia gibsoni cytochrome b Gene in Western Japan
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DOI：
10.1292/jvms.12-0140
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发表时间：
2012-10-01
影响因子：
1.2
通讯作者：
Endo, Yasuyuki
中科院分区：
文献类型：
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作者：
Sakuma, Masato;Fukuda, Kenta;Endo, Yasuyuki
本研究对吉氏巴氏巴氏鱼细胞色素B（cytB）基因（B. gibsoni），以确定潜在的阿托伐醌（ATV）抗性变体的流行。从自然B中采集92份血样。吉氏感染的狗。通过直接测序确定cytb核苷酸序列。在cytb中鉴定了12个非同义氨基酸替换。在三个病例中检测到主要的ATV抗性替换M1211。这项调查确定，潜在的ATV耐药B。吉氏菌株存在于日本的狗中。
In this study, we conducted a survey of the cytochrome b (cytb) gene of Babesia gibsoni (B. gibsoni) isolated from clinical cases to determine the prevalence of potential atovaquone (ATV)-resistant variants. Ninety-two blood samples were collected from naturally B. gibsoni infected dogs. The cytb nucleotide sequence was determined by direct sequencing. Twelve non-synonymous amino acid substitutions were identified in cytb. The principal ATV-resistant substitution, M1211, was detected in three cases. This survey determined that potentially ATV-resistant B. gibsoni strains are present in dogs in Japan.