Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana.
Induction of BiP by sugar independent of a cis-element for the unfolded protein response in Arabidopsis thaliana.
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DOI：
10.1016/j.bbrc.2006.05.189
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发表时间：
2006-08
影响因子：
3.1
通讯作者：
H. Tajima;N. Koizumi
中科院分区：
文献类型：
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作者：
H. Tajima;N. Koizumi
BiP是一种在未折叠蛋白反应(UPR)中诱导的分子伴侣。在哺乳动物细胞中，BIP是由葡萄糖饥饿诱导的，被称为葡萄糖调节蛋白78(GRP78)。然而，在拟南芥中，我们证明了Bip转录随着糖的耗尽而减少，随着糖的增加而增加。由BiP启动子驱动的β-葡萄糖醛酸苷酶(GUS)转录本对衣霉素和糖的反应类似于转基因拟南芥中内源BiP转录本。当GUS被P-UPRE调控时，GUS转录产物因糖饥饿而积累。P-UPRE是Bip启动子中负责UPR的顺式元件。随后对含有P-UPRE调控的荧光素酶(Luc)基因的转基因拟南芥进行了分析。糖的缺乏也增加了LUC的活性。结果表明，BIP是由糖诱导的，不依赖于负责UPR的顺式元件。
BiP is a molecular chaperone induced in the unfolded protein response (UPR). In mammalian cells, BiP is induced by glucose starvation when it is called glucose-regulated protein 78 (GRP78). In Arabidopsis thaliana, however, we demonstrated that BiP transcripts decreased with sugar depletion and increased with sugar addition. Transcripts for β-glucuronidase (GUS) driven by BiP promoter respond to tunicamycin and sugar, being similar with endogenous BiP transcripts in transgenic A. thaliana. When GUS was regulated by P-UPRE, a cis-element responsible for the UPR identified in BiP promoter, GUS transcripts were accumulated by sugar starvation. Subsequently, transgenic A. thaliana harboring luciferase (LUC) gene regulated by P-UPRE was analyzed. Sugar depletion also increased LUC activity. It is concluded that BiP is induced by sugar independent of the cis-element responsible for the UPR.