Kurozu melanoidin, a novel oligoglucan-melanoidin complex from Japanese black vinegar, suppresses adipogenesis in vitro.
Kurozu melanoidin, a novel oligoglucan-melanoidin complex from Japanese black vinegar, suppresses adipogenesis in vitro.
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Kurozu melanoidin 是一种来自日本黑醋的新型低聚葡聚糖-蛋白黑素复合物，可在体外抑制脂肪形成。
DOI：
10.1016/j.jff.2020.104046
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发表时间：
2020
期刊：
影响因子：
5.6
通讯作者：
Hasumi K
中科院分区：
文献类型：
--
作者：
Suzuki E;Otake S;Hamadate N;Hasumi K
黑醋“黑津”具有抗肥胖的潜力，但其有效原理尚不清楚。在这里，我们分离和表征一种新的低聚葡聚糖-类黑精复合物，命名为黑素（KZM），抑制体外脂肪生成。通过有机溶剂萃取，然后通过尺寸排阻色谱法分离KZM。使用聚乙二醇作为标准品，其平均分子量估计为1，090 ± 30 Da。光谱和化学分析的组合揭示了KZM中存在低聚葡聚糖和类黑精结构。葡聚糖部分主要由1 → 4键组成。MALDI-TOF-MS分析确定了一系列离子峰，其m/z相差约162.055 [C6 H12 O 6 − H2O]，表明葡聚糖链长的变化。在dm/z500-600附近的突出信号不能归属于葡聚糖单元，表明类黑精部分的离子峰。KZM在100 μM时显著抑制3 T3-L1前脂肪细胞的脂肪生成。
The black vinegar “kurozu” has an anti-obesity potential, but its active principle is unknown. Here, we isolate and characterize a novel oligoglucan-melanoidin complex, designated kurozu melanoidin (KZM), that suppresses adipogenesisin vitro. KZM was isolated by organic solvent extraction, followed by size-exclusion chromatography. Its average molecular mass was estimated to be 1,090 ± 30 Da using polyethylene glycols as standards. The combination of spectroscopic and chemical analyses revealed the presence of an oligoglucan and a melanoidin structures in KZM. The glucan moiety consisted mainly of 1 → 4 linkage. The MALDI-TOF-MS analysis identified an array of ion peaks that differed inm/zby ~ 162.055 [C6H12O6− H2O], indicating a variation of glucan chain length. Prominent signals aroundm/z500–600 that were unassignable to glucan units suggested ion peaks for the melanoidin moiety. KZM significantly suppressed adipogenesis of 3T3-L1 preadipocytes at 100 μM.