INVIVO IMAGING OF CALCIUM ACCUMULATION IN FLY INTERNEURONS AS ELICITED BY VISUAL-MOTION STIMULATION
INVIVO IMAGING OF CALCIUM ACCUMULATION IN FLY INTERNEURONS AS ELICITED BY VISUAL-MOTION STIMULATION
复制标题
DOI：
10.1073/pnas.89.9.4139
复制
发表时间：
1992-05-01
影响因子：
11.1
通讯作者：
EGELHAAF, M
中科院分区：
文献类型：
--
作者：
BORST, A;EGELHAAF, M
运动的计算在视觉定位中起着核心作用。苍蝇已被成功地用作模型系统，用于分析运动检测的机制。因此，人们对第三视觉神经节中可单独识别的神经元的神经元回路给予了很大的关注，该神经元回路提取不同类型的视网膜运动模式并将这些模式转换为视觉定向行为的特定成分。这些大细胞的扩展树突树是许多空间分布的局部运动敏感元件的会聚点。正如体内显微荧光成像所揭示的，这些细胞在视觉运动刺激激活期间积累钙。钙分布的时空模式显示了以下特征：（i）钙积累首先在空间上限于那些由视网膜定位输入去极化的树突分支，（ii）在持续的运动刺激期间，钙也可能在整个细胞中积累，特别是在不接受直接突触输入的区域。这些实验成功地监测活性依赖性离子在活体动物的视觉中间神经元的自然突触输入刺激的细胞内分布。
The computation of motion plays a central role in visual orientation. The fly has been successfully used as a model system for analyzing the mechanisms underlying motion detection. Thereby, much attention has been paid to a neuronal circuit of individually identifiable neurons in the third visual ganglion that extracts different types of retinal motion patterns and converts these patterns into specific components of visual orientation behavior. The extended dendritic trees of these large cells are the sites of convergence of numerous spatially distributed local motion-sensitive elements. As is revealed by in vivo microfluorometric imaging, these cells accumulate calcium during activation by visual motion stimulation. The spatiotemporal pattern of calcium distribution shows the following characteristics: (i) calcium accumulation is first spatially restricted to those dendritic branches that are depolarized by the retinotopic input, (ii) during ongoing motion stimulation calcium may also accumulate throughout the cell and, in particular, in regions that do not receive direct synaptic input. These experiments successfully monitor the intracellular distribution of activity-dependent ions in visual interneurons of living animals stimulated by their natural synaptic input.