MORPHOLOGICAL REVERSION OF SPIRULINA (ARTHROSPIRA) PLATENSIS (CYANOPHYTA): FROM LINEAR TO HELICAL 1
MORPHOLOGICAL REVERSION OF SPIRULINA (ARTHROSPIRA) PLATENSIS (CYANOPHYTA): FROM LINEAR TO HELICAL 1
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DOI：
10.1111/j.1529-8817.2005.00087.x
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发表时间：
2005-06
影响因子：
2.9
通讯作者：
Zhi Wang;Ying Zhao
中科院分区：
文献类型：
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作者：
Zhi Wang;Ying Zhao
蓝藻螺旋藻Turpin的特点是其规则卷曲的毛状体。在某些条件下，它的螺旋丝可以转化为异常的形态，如不规则的弯曲，甚至线性的形状，这被认为是一个永久性的变性，不能逆转。然而，在这里，我们发现螺旋藻的线性细丝可以自发地恢复到螺旋形式与原始细丝相同的形态。进一步的研究表明，线状花丝的超微结构、生理生化特性与原始花丝不同，而回复花丝与原始花丝的超微结构、生理生化特性相同。SDS-PAGE分析揭示了至少四种与螺旋藻形态发生相关的蛋白质或亚基：21.9-kDa和20.3-kDa蛋白质在螺旋藻丝中高度表达，而52.0-kDa和31.8-kDa蛋白质在线状藻丝中高度表达。用96个随机引物进行的随机扩增多态性DNA分析表明，回复丝的遗传背景与原丝相同，但与直丝不同。结果表明，螺旋藻的线状藻丝在一定条件下可以恢复原来的形态，并恢复其其他显著特征。
The cyanobacterium Spirulina Turpin is characterized by its regularly coiled trichomes. Under some conditions, its helical filaments can convert to abnormal morphologies, such as irregularly curved and even linear shapes, that had been considered as a permanent degeneration that could not be reversed. However, here we found that the linear filaments of Spirulina platensis Geitler could spontaneously revert to the helical form with the same morphology as the original filaments. Further studies showed that the ultrastructural, physiological, and biochemical characteristics of linear filaments were different from those of the original filaments, whereas they were the same for the reverted and the original filaments. The SDS‐PAGE analysis revealed at least four proteins or subunits related to Spirulina morphogenesis: The 21.9‐kDa and the 20.3‐kDa proteins were highly expressed in the helical filaments, whereas the 52.0‐kDa and the 31.8‐kDa proteins were highly expressed in the linear filaments. The random amplified polymorphic DNA analysis with 96 random primers showed that the genetic background of the reverted filaments was the same as that of the original filaments but distinct from that of the linear filaments. The results indicated that linear filaments of Spirulina could revert to the original morphology under certain conditions, and their other distinctive traits were regained.