Connective tissue activation. XVII. Radioimmunoassay of a human platelet derived connective tissue activating peptide (CTAP-III) and specificities of anti-CTAP-III sera.
Connective tissue activation. XVII. Radioimmunoassay of a human platelet derived connective tissue activating peptide (CTAP-III) and specificities of anti-CTAP-III sera.
复制标题
结缔组织激活。
DOI：
10.1016/0009-8981(80)90350-2
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发表时间：
1980
期刊：
影响因子：
--
通讯作者：
Anderson,B
中科院分区：
文献类型：
--
作者：
Weiss,JJ;Meyers,S;Castor,CW;Donakowski,C;Anderson,B
血小板衍生的结缔组织激活肽(CTAP-III)是刺激包括滑膜组织细胞在内的人结缔组织细胞株代谢和增殖的重要因素。CTAP-III影响细胞变化的量以及在各种生物液和组织中的量很少。本研究的目的是建立CTAP-III的放射免疫分析(RIA)，并确定抗CTAP-III血清试剂的特异性。抗血清被证明不与许多多肽激素发生交叉反应。然而，另外两种血小板蛋白，β-血栓球蛋白和低亲和力血小板因子-4，在放射免疫系统中与CTAP-III一样竞争抗CTAP-III抗体。因此，这三种血小板蛋白在构成反应性抗原决定簇的分子部分上是相似或相同的。正常人无血小板血浆中抑制抗CTAP-III-12S！-CTAP-III复合体形成的物质水平为34±13(S.D.)Ng/ml。
The platelet-derived connective tissue activating peptide (CTAP-III) has been shown to be an important factor stimulating the metabolism and proliferation of human connective tissue cell strains, including synovial tissue cells. The quantities of CTAP-III affecting the cellular changes and the amounts in various biologic fluids and tissues are small. The objectives of this study were to develop a radioimmunoassay (RIA) for CTAP-III and to ascertain the specificities of the anti-CTAP-III sera reagents. The antisera were shown not to cross-react with a number of polypeptide hormones. However, two other platelet proteins, β-thromboglobulin and low affinity platelet factor-4, competed equally as well as CTAP-III for anti-CTAP-III antibodies in the RIA system. Thus, the three platelet proteins are similar or identical with respect to those portions of the molecules constituting the reactive antigenic determinants. The levels of material in normal human platelet-free plasma that inhibited anti-CTAP-III-12S!-CTAP-III complex formation were determined to be 34 ± 13 (S.D.) ng/ml.