Visualizing postendocytic traffic of synaptic vesicles at hippocampal synapses
Visualizing postendocytic traffic of synaptic vesicles at hippocampal synapses
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DOI：
10.1016/s0896-6273(01)00398-1
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发表时间：
2001-08-30
期刊：
影响因子：
16.2
通讯作者：
Murthy, VN
中科院分区：
文献类型：
--
作者：
Li, ZY;Murthy, VN
我们已经研究了在postendocytic，在海马突触的突触囊泡加工机制，使用synaptobrevin/囊泡相关膜蛋白（VAMP）标记的变体的绿色荧光蛋白。胞吐作用后，VAMP在突触和相邻轴突区域被回收。回收的含VAMP的囊泡以比内吞作用慢的速率返回突触囊泡簇。囊泡含有不同的蛋白质，突触素，以类似的速度重新聚集，这表明两种蛋白质的共同囊泡中间体。活性是指内吞的小泡返回突触所需的时间。外源性钙缓冲剂减缓内吞作用，但对再聚集的时间过程没有额外的影响。相反，蛋白激酶抑制剂staurosporine不影响内吞作用，但减缓再聚集。最后，由于VAMP可以在表面膜上自由移动，持续的突触活动导致相邻突触之间的囊泡成分混合。
We have investigated mechanisms in postendocytic, processing of synaptic vesicles at hippocampal synapses, using synaptobrevin/vesicle-associated membrane protein (VAMP) tagged with variants of the green fluorescent protein. Following exocytosis, VAMP is retrieved at synaptic and adjoining axonal regions. Retrieved VAMP-containing vesicles return to synaptic vesicle clusters at a rate slower than endocytosis. Vesicles containing a different protein, synaptophysin, recluster at a similar rate, suggesting common vesicular intermediates for the two proteins. Activity prolongs the time taken by endocytosed vesicles to return to synapses. Exogenous calcium buffers slow endocytosis but have no additional effect on the time course of reclustering. In contrast, the protein kinase inhibitor staurosporine does not affect endocytosis but slows reclustering. Finally, since VAMP can move freely on surface membranes, sustained synaptic activity leads to mixing of this vesicular component between adjacent synapses.