The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster.
The corticotropin-releasing factor-like diuretic hormone 44 (DH44) and kinin neuropeptides modulate desiccation and starvation tolerance in Drosophila melanogaster.
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DOI：
10.1016/j.peptides.2016.02.004
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发表时间：
2016-06
期刊：
影响因子：
3
通讯作者：
Davies SA
中科院分区：
文献类型：
--
作者：
Cannell E;Dornan AJ;Halberg KA;Terhzaz S;Dow JAT;Davies SA
CRF样利尿激素44（DH 44）信号调节D.黑腹菌D.黑腹动物激肽（Drome-kinin，DK）在饥饿胁迫耐受中具有新的作用。DH 44和激肽信号通路之间存在功能性相互作用。马氏管是昆虫上皮细胞液体运输和胁迫耐受的重要器官，受神经元分泌的多种神经肽的神经内分泌调控。在这里，我们证明CRF样利尿激素44（DH 44）和果蝇激肽（Drome-kinin，DK）在干燥和饥饿耐受中的作用。基因表达和标记的DH 44配体结合数据，以及高度选择性敲低和/或神经元消融的大脑间部的神经元和DH 44受体（DH 44-R2）在马氏管主细胞的DH 44，表明DH 44信号的抑制提高了干燥耐受性的完整的苍蝇。由白细胞激肽受体基因LKR编码的Drome-kinin受体在DH 44神经元以及马氏管的星状细胞中表达。在表达DH 44的神经元中的LKR敲低降低了马氏管特异性LKR，表明DH 44和LK信号传导通路之间的相互作用。最后，虽然DK在脱水耐受性中的作用尚未确定，但我们证明了马氏管细胞特异性LKR在饥饿耐受性中的新作用。饥饿增加上皮LKR的基因表达。此外，马氏管星状细胞特异性敲低LKR显着降低饥饿耐受性，表明在饥饿应激过程中的神经肽信号转导的作用。
CRF-like diuretic hormone 44 (DH44) signalling modulates desiccation tolerance in D. melanogaster. D. melanogaster kinin (Drome-kinin, DK) has a novel role in starvation stress tolerance. There are functional interactions between DH44 and kinin signalling pathways. Malpighian tubules are critical organs for epithelial fluid transport and stress tolerance in insects, and are under neuroendocrine control by multiple neuropeptides secreted by identified neurons. Here, we demonstrate roles for CRF-like diuretic hormone 44 (DH44) and Drosophila melanogaster kinin (Drome-kinin, DK) in desiccation and starvation tolerance. Gene expression and labelled DH44 ligand binding data, as well as highly selective knockdowns and/or neuronal ablations of DH44 in neurons of the pars intercerebralis and DH44 receptor (DH44-R2) in Malpighian tubule principal cells, indicate that suppression of DH44 signalling improves desiccation tolerance of the intact fly. Drome-kinin receptor, encoded by the leucokinin receptor gene, LKR, is expressed in DH44 neurons as well as in stellate cells of the Malpighian tubules. LKR knockdown in DH44-expressing neurons reduces Malpighian tubule-specific LKR, suggesting interactions between DH44 and LK signalling pathways. Finally, although a role for DK in desiccation tolerance was not defined, we demonstrate a novel role for Malpighian tubule cell-specific LKR in starvation tolerance. Starvation increases gene expression of epithelial LKR. Also, Malpighian tubule stellate cell-specific knockdown of LKR significantly reduced starvation tolerance, demonstrating a role for neuropeptide signalling during starvation stress.