Structure of transcripts from the homeotic Antennapedia gene of Drosophila melanogaster: two promoters control the major protein-coding region.
Structure of transcripts from the homeotic Antennapedia gene of Drosophila melanogaster: two promoters control the major protein-coding region.
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果蝇同源触角足基因的转录物结构：两个启动子控制主要蛋白质编码区。
DOI：
10.1128/mcb.6.12.4676-4689.1986
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发表时间：
1986
影响因子：
5.3
通讯作者：
Scott,MP
中科院分区：
文献类型：
--
作者：
Laughon,A;Boulet,AM;BerminghamJr,JR;Laymon,RA;Scott,MP
黑腹果蝇的触角足（Antp）同源异型基因调节胸部的节段同一性。 Antp功能的丧失会导致胚胎胸节的发育改变，或者可能导致腿转变为触角。蚂蚁进动致死等位基因的某些组合互补以允许正常发育。通过对 cDNA 克隆和外显子进行测序以及转录本作图分析，对 Antp 基因的结构进行了分析，揭示了其遗传复杂性的一些基础。它有两个启动子，控制两个嵌套转录单元，一个单元长 36，另一个单元长 103 千碱基对 (kb)。两个单位都包含相同的蛋白质编码外显子，所有外显子都位于基因的 3' 最端 13 kb。两个启动子导致两个长非编码前导序列（1.5 和 1.7 kb）中的任何一个连接到 1.1 kb 开放阅读框。两个转录单位都使用同一对相距 1.4 kb 的替代多聚腺苷酸化位点；地点的选择是受发展调节的。一些破坏较大转录单位的突变与影响较小转录单位的突变互补。将触角转变为腿的显性突变分裂了基因，但编码外显子保持完整。编码的蛋白质具有异常长的谷氨酰胺序列和 C 末端附近的同源结构域。
The Antennapedia (Antp) homeotic gene ofDrosophila melanogasterregulates segmental identity in the thorax. Loss ofAntpfunction results in altered development of the embryonic thoracic segments or can cause legs to be transformed into antennae. Certain combinations ofAntprecessive lethal alleles complement to permit normal development. The structure of theAntpgene, analyzed by sequencing cDNA clones and exons and by transcript mapping, revealed some of the basis for its genetic complexity. It has two promoters governing two nested transcription units, one unit 36 and one 103 kilobase pairs (kb) long. Both units incorporated the same protein-coding exons, all of which are located in the 3′-most 13 kb of the gene. The two promoters resulted in the attachment of either of two long noncoding leader sequences (1.5 and 1.7 kb) to a 1.1-kb open reading frame. Both transcription units used the same pair of alternative polyadenylation sites 1.4 kb apart; the choice of sites was developmentally regulated. Some of the mutations that disrupt the larger transcription unit complemented a mutation affecting the smaller one. Dominant mutations that transform antennae into legs split the gene but left the coding exons intact. The encoded protein has unusually long runs of glutamine and a homeodomain near the C terminus.