Evaluation of ion release and the recharge ability of glass-ionomer cement containing BioUnion filler using an in vitro saliva-drop setting assembly
Evaluation of ion release and the recharge ability of glass-ionomer cement containing BioUnion filler using an in vitro saliva-drop setting assembly
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DOI：
10.1016/j.dental.2021.02.022
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发表时间：
2021-04-17
期刊：
影响因子：
5
通讯作者：
Imazato, Satoshi
中科院分区：
文献类型：
--
作者：
Kohno, Tomoki;Liu, Yuhan;Imazato, Satoshi
目标。一种含有BioUnion填料的玻璃离子水门汀(GIC)被报道在酸性条件下释放锌离子，并抑制其表面的口腔细菌。然而，以前的结果是基于静态条件下的体外实验。本研究旨在组装一种模拟口腔活体环境的体外唾液滴定器，并研究含有BioUnion填充剂的GIC的离子释放和充电特性。测定了锌和氟对变形链球菌和唾液衍生多菌种生物膜的有效浓度。使用体外唾液滴定组件将人工唾液滴在含有BioUnion填充物的GIC上，并定期用醋酸替换。通过测定锌离子和氟离子的释放浓度来研究离子的释放/充电特性。含有BioUnion填料的GIC在反复暴露于酸的7天内释放的锌离子浓度可以保持在抑制变形链球菌和唾液衍生的多物种生物膜形成的水平。此外，含有GIC的BioUnion填料可以通过使用含锌和F-的牙胶进行锌和F-的充电。充电后锌离子的释放浓度明显高于锌离子的有效浓度，对抑制变形链球菌和唾液衍生的多物种生物膜在材料表面的形成具有重要意义。含有BioUnion填充物的GIC被证明具有抑制口腔内生物膜形成的潜力。此外，对含有BioUnion填料的GIC进行锌离子和氟离子的充填将进一步增强其效果。(C)2021年香港牙科材料学院。爱思唯尔公司出版，版权所有。
Objective. A glass-ionomer cement (GIC) containing BioUnion filler has been reported to release Zn2+ under acidic conditions and to inhibit oral bacteria on its surface. However, previous results are based on in vitro experiments under static conditions. This study aimed to assemble an in vitro saliva-drop setting to simulate in vivo conditions of the oral cavity and to investigate the ion releasing and recharging properties of the GIC containing BioUnion filler.Methods. The effective concentrations of Zn2+ and F- against Streptococcus mutans and saliva derived multi-species biofilms were determined. Artificial saliva was dropped on the GIC containing BioUnion filler using the in vitro saliva-drop setting assembly and was periodically replaced with acetic acid. Ion release/recharge properties were investigated by measuring the release concentrations of Zn2+ and F-.Results. The concentration of Zn2+ released from the BioUnion filler-containing GIC during seven days with repeated exposure to acid could be maintained at the level to inhibit S. mutans and saliva-derived multi-species biofilm formation. Moreover, the BioUnion filler containing GIC could be recharged with Zn2+ and F- by the application of a tooth gel containing Zn2+ and F-. The release concentration of Zn2+ after recharging was significantly higher than the effective concentration of Zn2+ to hinder S. mutans and saliva-derived multi-species biofilm formation on material surfaces.Significance. The GIC containing BioUnion filler was shown to have the potential to inhibit biofilm formation in the oral cavity. In addition, recharging Zn2+ and F- would further enhance the effect of the GIC containing BioUnion filler. (C) 2021 The Academy of Dental Materials. Published by Elsevier Inc. All rights reserved.