ANTIBODY REDESIGN BY CHAIN SHUFFLING FROM RANDOM COMBINATORIAL IMMUNOGLOBULIN LIBRARIES
ANTIBODY REDESIGN BY CHAIN SHUFFLING FROM RANDOM COMBINATORIAL IMMUNOGLOBULIN LIBRARIES
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DOI：
10.1073/pnas.88.24.11120
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发表时间：
1991-12-01
影响因子：
11.1
通讯作者：
BURTON, DR
中科院分区：
文献类型：
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作者：
KANG, AS;JONES, TM;BURTON, DR
许多实验改组的重链和轻链从抗体的定义的过渡态类似物半抗原nitrophenyl phosphonamidate的特异性进行了说明。实验报告了结合抗原中重链和轻链的混杂性以及通过这种改组过程重新设计抗体的可行性。乱伦和无性系外滥交协会的概念进行了说明。改组打开了产生具有相关特异性但具有不同独特型组成的抗体组的可能性，其可能在治疗中使用人单克隆抗体中具有重要意义。
A number of experiments on the shuffling of heavy and light chains from antibodies of defined specificity for the transition-state analogue hapten nitrophenyl phosphonamidate are described. The experiments report on the promiscuity of heavy and light chains in binding antigen and the feasibility of antibody redesign by this shuffling process. The concepts of incestuous and extraclonal promiscuous association are described. Shuffling opens the possibility of generating panels of antibodies with related specificity but of distinct idiotypic composition that may have significance in the use of human monoclonal antibodies in therapy.