Mosaic distribution of chondroitin and keratan sulphate in the developing rat striatum: possible involvement of proteoglycans in the organization of the nigrostriatal system
Mosaic distribution of chondroitin and keratan sulphate in the developing rat striatum: possible involvement of proteoglycans in the organization of the nigrostriatal system
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DOI：
10.1016/s0165-3806(98)00088-1
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发表时间：
1998-08-08
期刊：
影响因子：
--
通讯作者：
Saxod, R
中科院分区：
文献类型：
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作者：
Charvet, I;Hemming, FJ;Saxod, R
哺乳动物基底神经节的纹状体由两个神经化学上不同的区室组成，称为斑块和基质，它们总体上构成了镶嵌组织。糖胺聚糖 (GAG) 是蛋白聚糖的糖部分，在多种功能神经系统的发育过程中提供特定的时空指导线索。然而，它们在黑质纹状体系统内的分布尚未得到研究。在这里，检查了大鼠发育中的新纹状体中未硫酸化（COS）、4-硫酸化（C4S）和6-硫酸化软骨素（C6S）和硫酸角质素（KS）的免疫组织化学分布，并与多巴胺能末端的分布进行比较。所有硫酸软骨素 (CS) 异构体均在胚胎纹状体中均匀表达。出生后，COS 和 C6S 显示纹状体嵌合体优先在基质区室和斑块周围的边界中表达，而 C4S 表位存在于两个区室中，具有轻微的斑片状分布。 KS 表达首先在出生后早期的斑块中检测到，随后仅在基质隔室中检测到。随着大脑成熟，所有这些 GAG 表达都会消失，但 C4S 除外，C4S 在整个成年生活中仍保持较高水平。此外，对发育中的内侧前脑束的研究表明，CS 异构体（而非 KS）在多巴胺轴突束内和周围表达，但显示出相似的发育分布模式，似乎与黑质纹状体通路没有特异性相关。这些结果表明蛋白多糖在纹状体发育过程中可能存在的意义，并且可能有助于理解帕金森病黑质纹状体回路变性和可塑性中复杂的细胞和分子相互作用。 (C) 1998 Elsevier Science B.V. 保留所有权利。
The striatum of the mammalian basal ganglia is composed of two neurochemically distinct compartments termed patches and matrix that contribute overall to a mosaic organization. Glycosaminoglycans (GAGs), the sugar moieties of proteoglycans, provide specific spatio-temporal guidance cues during the development of several functional neural systems. However, their distribution within the nigrostriatal system has not been investigated yet. Here, the immunohistochemical distributions of unsulphated (COS), 4-sulphated (C4S) and 6-sulphated chondroitin (C6S) and keratan sulphate (KS) were examined in the developing neostriatum of rat and compared with the distribution of dopaminergic terminals. All the chondroitin sulphate (CS) isomers are homogeneously expressed in the embryonic striatum. After birth, COS and C6S reveal the striatal mosaic in being preferentially expressed within the matrix compartment and in boundaries around patches whereas the C4S epitope is present in both compartments, with a slight patchy distribution. KS expression is detected first in the patches during the early postnatal period and subsequently only in the matrix compartment. All these GAG expressions disappear as the brain matures except for C4S which remains high throughout adult life. Furthermore, studies within the developing medial forebrain bundle reveal that CS isomers, but not KS, are expressed in and around the dopamine axonal tract but show similar developmental patterns of distribution which do not appear to be specifically associated with the nigrostriatal pathway. These results suggest a possible implication of proteoglycans during the development of the striatum and may be useful for understanding the complex cellular and molecular interactions in degeneration and plasticity of the nigrostriatal circuit in Parkinson's disease. (C) 1998 Elsevier Science B.V. All rights reserved.