Hierarchy of elements regulating synthesis of ribosomal proteins in Saccharomyces cerevisiae.
Hierarchy of elements regulating synthesis of ribosomal proteins in Saccharomyces cerevisiae.
复制标题
酿酒酵母中调节核糖体蛋白合成的元素的层次结构。
DOI：
10.1128/mcb.1.11.1016-1023.1981
复制
发表时间：
1981
影响因子：
5.3
通讯作者：
Warner,JR
中科院分区：
文献类型：
--
作者：
Kief,DR;Warner,JR
酵母细胞对热休克的反应是暂时减缓核糖体蛋白的合成（C。Gorenstein和J.R. Warner，Proc. Natl. Acad. Sci. U.S.A.73：1574-1551，1976）。当以乙醇为唯一碳源的氧化性培养物从23 ° C移至36°C时，核糖体蛋白质的合成受到协同抑制，其速度是以葡萄糖为唯一碳源的发酵性培养物的两倍，严重程度为45%。在15分钟内，至少30种核糖体蛋白质的相对合成速率下降到不到其初始值的六分之一，而蛋白质合成的总体速率至少增加了三倍。我们认为，这主要是由于在核糖体蛋白质的信使核糖核酸的合成水平的控制，但也可能涉及信使核糖核酸的稳定性的变化。与此相反，营养向上转移会刺激核糖体蛋白的合成。旨在确定影响这些蛋白质合成的刺激的层次结构的实验表明，温度冲击是占主导地位的葡萄糖刺激。当乙醇培养基的温度从23 °C升至36°C，随后加入葡萄糖时，核糖体蛋白质合成的下降趋势丝毫没有减弱。然而，在野生型细胞中，核糖体蛋白质合成在15分钟内开始恢复。rna 2的产物在大多数核糖体蛋白质的合成中没有恢复，这表明rna 2的产物在所有营养条件下对于这些蛋白质的产生是必需的。
Saccharomyces cerevisiaecells respond to a heat shock by temporarily slowing the synthesis of ribosomal proteins (C. Gorenstein and J. R. Warner, Proc. Natl. Acad. Sci. U.S.A.73:1574–1551, 1976). When cultures growing oxidatively on ethanol as the sole carbon source were shifted from 23 to 36°C, the synthesis of ribosomal proteins was coordinately inhibited twice as rapidly and 45% more severely than in comparable cultures growing fermentatively on glucose. Within 15 min, the relative rates of synthesis of at least 30 ribosomal proteins declined to less than one-sixth their initial values, whereas the overall rate of protein synthesis increased at least threefold. We suggest that this is due primarily to controls at the level of synthesis of messenger ribonucleic acid for ribosomal proteins but may also involve changes in messenger ribonucleic acid stability. In contrast, a nutritional shift-up causes a stimulation of the synthesis of ribosomal proteins. Experiments designed to determine the hierarchy of stimuli affecting the synthesis of these proteins demonstrated that temperature shock was dominant to glucose stimulation. When a culture growing on ethanol was shifted from 23 to 36°C and glucose was added shortly afterward, the decline in ribosomal protein synthesis continued unabated. However, in wild-type cells ribosomal protein synthesis began to recover within 15 min. In mutants temperature sensitive for ribosome synthesis, e.g.,rna2, there was no recovery in the synthesis of most ribosomal proteins, suggesting that the product ofrna2is essential for the production of these proteins under all vegetative conditions.