Fusogenic activity of reconstituted newcastle disease virus envelopes:: a role for the hemagglutinin-neuraminidase protein in the fusion process
Fusogenic activity of reconstituted newcastle disease virus envelopes:: a role for the hemagglutinin-neuraminidase protein in the fusion process
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DOI：
10.1016/s1357-2725(01)00127-3
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发表时间：
2002-04-01
影响因子：
4
通讯作者：
Villar, E
中科院分区：
文献类型：
--
作者：
Cobaleda, C;Muñoz-Barroso, I;Villar, E
有包膜的病毒，如纽卡斯尔疫病毒（NDV），通过膜融合进入宿主细胞。在NDV的情况下，融合步骤需要跨膜血凝素-神经氨酸酶（HN）和融合（F）病毒包膜糖蛋白。HN蛋白应显示融合促进活性。到目前为止，HN-F相互作用L的性质是一个有争议的问题。在这项工作中，我们的目标是澄清HN糖蛋白在膜融合步骤中的作用。使用四种类型的重构无去污剂NDV包膜，不同的包膜蛋白含量。使用十八烷基罗丹明B氯化物测定法监测不可逆病毒体和红细胞血影的融合。即使在不存在HN蛋白的情况下，仅具有F蛋白的重构包膜显示出残余的融合活性。用影响F蛋白的变性剂处理这样的病毒体废除融合，表明检测到的融合是病毒蛋白依赖的。有趣的是，在存在FIN唾液酸酶活性抑制剂2，3-双氧-2-脱氧-N-乙酰神经氨酸的情况下，重组系统中的融合率与完整病毒的融合率相似。结果表明，在重构系统中检测到的残留融合活性完全是由于F蛋白活性，而没有HN蛋白的融合促进活性的贡献。(C)2002爱思唯尔科技有限公司。保留所有权利。
Enveloped viruses, such as newcastle disease virus (NDV), make their entry into the host cell by membrane fusion. In the case of NDV, the fusion step requires both transmembrane hemagglutinin-neuraminidase (HN) and fusion (F) viral envelope glycoproteins. The HN protein should show fusion promotion activity. To date, the nature of HN-F interactions L is a controversial issue. In this work, we aim to clarify the role of the HN glycoprotein in the membrane fusion step. Four types of reconstituted detergent-free NDV envelopes were used, on differing in their envelope protein contents. Fusion of the differerent virosomes and erythrocyte ghosts was monitored using the octadecyl rhodamine B chloride assay. Only the reconstituted envelopes having the F protein, even in the absence of HN protein, displayed residual fusion activity. Treatment of such virosomes with denaturing agents affecting the F protein abolished fusion, indicating that the fusion detected was viral protein-dependent. Interestingly, the rate of fusion in the reconstituted systems was similar to that of intact viruses in the presence of the inhibitor of FIN sialidase activity 2,3-dehydro-2-deoxy-N-acetylneuraminic acid. The results show that the residual fusion activity detected in the reconstituted systems was exclusively due to F protein activity, with no contribution from the fusion promotion activity of HN protein. (C) 2002 Elsevier Science Ltd. All rights reserved.