Identification of Armillaria field isolates using isozymes and mycelial growth characteristics
Identification of Armillaria field isolates using isozymes and mycelial growth characteristics
复制标题
利用同工酶和菌丝生长特征鉴定蜜环菌野外分离株
DOI：
10.1023/a:1006998109169
复制
发表时间：
2004
期刊：
影响因子：
5.5
通讯作者：
J. Bruhn
中科院分区：
文献类型：
--
作者：
J. Bruhn
本研究的目的是开发基于菌丝生长特征以及扩散菌丝体产生酯酶 (EST) 和多酚氧化酶 (PPO) 模式的程序，用于鉴定来自奥扎克山脉（美国中部）的栎树、山核桃和松树森林中的蜜环菌野外分离株。收集的 285 个分离株首先通过标准二倍体-单倍体配对测试鉴定为高卢放线菌、蜜环菌或瘰疬放线菌。强 PPO 条带可诊断高卢蘑菇。根据三个标准化 R f 范围内 EST 带的产生来区分所有测试的蜜环菌分离株和 91% 的测试的 A. tabescens 分离株。基于 24 °C 的单宁酸培养基 (TA) 和 33 °C 的麦芽提取物培养基 (ME) 上的菌丝生长和形态的程序正确识别了 98% 的高卢曲霉分离株以及所有蜜霉和烟曲霉分离株。在 TA 上，A. gallica 生长最慢。在 ME 上，蜜霉生长最慢：菌丝形态因物种而异；高卢曲霉通常对琼脂染色，并产生贴压/浸没式生长模式，具有菌丝密度递减的同心带；蜜霉通常不对琼脂染色，产生具有光滑边缘和丰富气生菌丝的圆形菌丝体；烟曲霉通常对琼脂染色，并以非常不规则的边缘和斑片状菌丝密度贴压/浸没式生长。这些是第一个发表的系统，根据菌丝生长特征以及 EST 和 PPO 补充来评估识别蜜环菌田间分离株的潜力。
This research was conducted to develop procedures based on mycelial growth characteristics and patterns of esterase (EST) and polyphenol oxidase (PPO) production by diffuse mycelia for identification of Armillaria field isolates from Quercus-Carya-Pinus forests in the Ozark Mountains (central USA). The 285 isolates collected were first identified by standard diploid-haploid pairing tests as A. gallica, A. mellea, or A. tabescens. A strong PPO band was diagnostic for A. gallica. All A. mellea isolates tested and 91% of the A. tabescens isolates tested were distinguished based on production of EST bands in three standardized R f ranges. A procedure based on mycelial growth and morphology on tannic acid medium (TA) at 24 °C and on malt extract medium (ME) at 33 °C correctly identified 98% of A. gallica isolates and all A. mellea and A. tabescens isolates. On TA, A. gallica grew slowest. On ME, A. mellea grew slowest: mycelial morphology differed among species; A. gallica typically stained the agar and produced an appressed/submerged growth pattern with concentric bands of decreasing hyphal density, A. mellea typically did not stain the agar and produced round mycelia with smooth margins and abundant aerial hyphae, A. tabescens typically stained the agar and grew appressed/submerged with very irregular margins and patchy hyphal density. These are the first published systems evaluating the potential for identifying Armillaria field isolates based on their mycelial growth characteristics and EST and PPO complements.