SEQUENCES OF THE EPSTEIN-BARR-VIRUS (EBV) LARGE INTERNAL REPEAT FORM THE CENTER OF A 16-KILOBASE-PAIR PALINDROME OF EBV (P3HR-1) HETEROGENEOUS DNA
SEQUENCES OF THE EPSTEIN-BARR-VIRUS (EBV) LARGE INTERNAL REPEAT FORM THE CENTER OF A 16-KILOBASE-PAIR PALINDROME OF EBV (P3HR-1) HETEROGENEOUS DNA
复制标题
DOI：
10.1128/jvi.61.5.1495-1506.1987
复制
发表时间：
1987-05-01
影响因子：
5.4
通讯作者：
MILLER, G
中科院分区：
文献类型：
--
作者：
JENSON, HB;FARRELL, PJ;MILLER, G
我们先前已经表征了P3 HR-1（HR-1）系所携带的一个缺陷EBV基因组中包含的EB病毒（EBV）DNA的几种基因组重排（H. B。Jenson，M. S. Rabson和G.米勒，J.病毒。58：475-486，1986）。来自该缺陷基因组的异源DNA（het DNA）的一个重组克隆是回文序列的16个内切酶对（kbp）的EcoRI片段。在含有het DNA的细胞中存在对该回文结构中心特异的DNA消化片段，但在缺乏het DNA的细胞中不存在。因此，回文不是DNA克隆的产物。通过DNA测序研究了这个回文序列中心的组织。还通过DNA测序研究了亲本HR-1基因组的可比区域。回文序列的中心3，495个碱基对（bp）由专门来自EBV内部重复1的序列组成，由BamHI W片段表示。在中心3，495 bp的每一端是与标准EBV基因组上超过50 kbp的BamHI-Z序列的对称重组。中间的3,495 bp由一个未重复的341 bp组成，两侧是两个1,577 bp的完美回文重复序列。341-bp独特区域是标准HR-1 BamHI-W的387-bp区域的一部分，其与回文序列的中心387 bp相同。这个中央387 bp的区域包含许多二分体对称的延伸，能够形成一个大的茎环结构。回文重排在来自标准HR-1 BamHI-W序列的het DNA中产生了两个新的开放阅读框架。这两个het DNA开放阅读框具有不同的氨基末端，但相同的羧基末端衍生自标准HR-1 BamHI-W（HR-1 BWRF 1）中的大开放阅读框。在het DNA中发现的BamHI-W序列既不包括标准HR-1 BamHI-W的TATA盒，也不包括编码EBV核抗原的潜在多顺反子潜伏mRNA中存在的外显子。基因组结构的这些显著改变可能与含有het DNA的病毒原液的独特生物学性质有关，其通过产生新的多肽或通过形成或缺失调节或功能信号来实现。
We have previously characterized several genomic rearrangements of Epstein-Barr virus (EBV) DNA contained in one of the defective EBV genomes harbored by the P3HR-1 (HR-1) line (H. B. Jenson, M. S. Rabson, and G. Miller, J. Virol. 58:475-486, 1986). One recombinant clone of heterogeneous DNA (het DNA) from this defective genome is an EcoRI fragment of 16 kilobase pairs (kbp) which is a palindrome. DNA digestion fragments specific for the center of this palindrome were present in cells which contained het DNA but not in cells which lacked het DNA. Thus, the palindrome was not an artifact of DNA cloning. The organization of the center of this palindrome was studied by DNA sequencing. The comparable region of the parental HR-1 genome was also studied by DNA sequencing. The central 3,495 base pairs (bp) of the palindrome were composed of sequences derived exclusively from internal repeat 1 of EBV, represented by the BamHI W fragment. At each end of the central 3,495 bp was a symmetrical recombination with sequences of BamHI-Z, located more than 50 kbp away on the standard EBV genome. The central 3,495 bp were composed of an unduplicated 341 bp flanked by two perfect palindromic repeats of 1,577 bp. The 341-bp unique region was a portion of a 387-bp region of standard HR-1 BamHI-W which was identical to the central 387 bp of the palindrome. This central 387-bp region contained numerous stretches of dyad symmetry capable of forming a large stem-and-loop structure. The palindromic rearrangement had created two novel open reading frames in het DNA derived from standard HR-1 BamHI-W sequences. These two het DNA open reading frames had different amino termini but identical carboxy termini derived from the large open reading frame in standard HR-1 BamHI-W (HR-1 BWRF1). The BamHI-W sequences found in het DNA did not include either the TATA box of standard HR-1 BamHI-W or the exons which are present inthe potentially polycistronic latent mRNAs encoding EBV nuclear antigens. These marked alterations in genomic structure may relate to the unique biologic properties of virus stocks containing het DNA by creation of new polypeptides or by formation or deletion of regulatory or functional signals.