Increased Susceptibility to Diet-Induced Obesity in Histamine-Deficient Mice
Increased Susceptibility to Diet-Induced Obesity in Histamine-Deficient Mice
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DOI：
10.1159/000095339
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发表时间：
2006-01-01
影响因子：
4.1
通讯作者：
Kjaer, Andreas
中科院分区：
文献类型：
--
作者：
Jorgensen, Emilie A.;Vogelsang, Thomas W.;Kjaer, Andreas
背景和目的：神经递质组胺参与小鼠食欲的调节和年龄相关性肥胖的发生。此外，组胺是瘦素促肾上腺皮质激素作用的介质。本研究的目的是探讨组胺在高脂饮食（HFD）诱导的肥胖症的发展中的可能作用。研究方法：给予组胺缺乏型组氨酸脱羧酶敲除（HDC-KO）小鼠和C57 BL/6 J野生型（WT）小鼠标准饮食（STD）或HFD 8周。测定大鼠体重、24 h热量摄入量、附睾脂肪组织大小、血浆瘦素浓度及瘦素受体（Ob-R）mRNA的定量表达。结果：HDC-KO和WT小鼠喂食HFD 8周后，其体重的增加显著高于STD喂食小鼠。喂食HFD或STD的HDC-KO小鼠在2周后观察到体重增加的显著差异，而WT小鼠在5周后首次观察到体重增加的显著差异。8周后，HFD小鼠的24小时热量摄入显著低于STD喂养的WT小鼠。在HDC-KO小鼠中，在HFD和STD喂养的小鼠之间没有观察到热量摄入的差异。8周后，附睾脂肪组织的大小和血浆瘦素浓度显着增加，在HFD喂养的WT和HDC-KO小鼠相比，其STD喂养的控制。HDC-KO小鼠的附睾脂肪组织大小高于WT小鼠，STD和HFD喂养小鼠均如此。观察到与STD喂养的HDC-KO小鼠相比，HFD喂养的HDC-KO小鼠中Ob-R mRNA显著降低，而在WT小鼠中未观察到这种差异。结论：根据我们的结果，我们得出结论，组胺在HFD诱导的肥胖的发展中起作用。版权所有（C）2006 S. Karger AG，巴塞尔
Background and Aim: The neurotransmitter histamine is involved in the regulation of appetite and in the development of age-related obesity in mice. Furthermore, histamine is a mediator of the anorexigenic action of leptin. The aim of the present study was to investigate a possible role of histamine in the development of high-fat diet (HFD)-induced obesity. Methods: Histamine-deficient histidine decarboxylase knock-out (HDC-KO) mice and C57BL/6J wild-type (WT) mice were given either a standard diet (STD) or HFD for 8 weeks. Body weight, 24-hour caloric intake, epididymal adipose tissue size, plasma leptin concentration and quantitative expression of leptin receptor (Ob-R) mRNA were measured. Results: Both HDC-KO and WT mice fed an HFD for 8 weeks increased their body weight significantly more than STD-fed mice. A significant difference in body weight gain between HDC-KO mice fed an HFD or an STD was seen after 2 weeks, whereas a significant difference in body weight gain was first observed after 5 weeks in WT mice. After 8 weeks 24-hour caloric intake was significantly lower in HFD- than in STD-fed WT mice. In HDC-KO mice no difference in caloric intake was observed between HFD- and STD-fed mice. After 8 weeks epididymal adipose tissue size and plasma leptin concentration had increased significantly in HFD-fed WT and HDC-KO mice compared to their STD-fed controls. Epididymal adipose tissue size was higher in HDC-KO than WT mice, both in STD-and HFD-fed mice. A significant decrease in Ob-R mRNA in HFD-fed HDC-KO mice compared to STD-fed HDC-KO mice was observed, while no such difference was observed in WT mice. Conclusion: Based on our results, we conclude that histamine plays a role in the development of HFD-induced obesity. Copyright (C) 2006 S. Karger AG, Basel