Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid.
Expansion microscopy provides new insights into the cytoskeleton of malaria parasites including the conservation of a conoid.
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扩张显微镜为疟疾寄生虫的细胞骨架提供了新的见解，包括圆锥体的守恒。
DOI：
10.1371/journal.pbio.3001020
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发表时间：
2021-03
期刊：
影响因子：
9.8
通讯作者：
Hamel V
中科院分区：
文献类型：
--
作者：
Bertiaux E;Balestra AC;Bournonville L;Louvel V;Maco B;Soldati-Favre D;Brochet M;Guichard P;Hamel V
疟疾是由单细胞疟原虫引起的。疟原虫依靠不同的微管细胞骨架结构进行繁殖、增殖和传播。由于这种寄生虫的小尺寸，其细胞骨架主要通过电子显微镜（EM）观察到。在这里，我们证明了纳米级的细胞骨架组织是触手可及的使用超微结构扩展显微镜（U-ExM）。在发育中的小配子体，U-ExM允许监测轴丝的动态组装和伴随的微管蛋白多聚谷氨酰胺化在整个细胞。在侵入性裂殖子和动合子形式中，U-ExM揭示了疟原虫阶段和赋予细胞刚性的膜下微管阵列物种的多样性。在动合子，我们还确定了一个顶端微管蛋白环（ATR），colocalises相关apicomplexan寄生虫的圆锥体的标记。这种含有微管蛋白的结构被认为是在疟原虫中丢失的，尽管它在其他顶复门动物的运动和入侵中起着关键作用。在这里，U-ExM揭示了一个发散的和相当减少的圆锥体形式实际上是保守的疟原虫物种。一项对疟原虫（导致疟疾的寄生虫）的扩增显微镜研究揭示了新的细胞骨架特征，包括残留的圆锥体-一种微管蛋白结构，尽管它在相关的顶复门寄生虫入侵宿主细胞中起着至关重要的作用，但据推测在这些生物体中丢失了这种结构。
Malaria is caused by unicellular Plasmodium parasites. Plasmodium relies on diverse microtubule cytoskeletal structures for its reproduction, multiplication, and dissemination. Due to the small size of this parasite, its cytoskeleton has been primarily observable by electron microscopy (EM). Here, we demonstrate that the nanoscale cytoskeleton organisation is within reach using ultrastructure expansion microscopy (U-ExM). In developing microgametocytes, U-ExM allows monitoring the dynamic assembly of axonemes and concomitant tubulin polyglutamylation in whole cells. In the invasive merozoite and ookinete forms, U-ExM unveils the diversity across Plasmodium stages and species of the subpellicular microtubule arrays that confer cell rigidity. In ookinetes, we additionally identify an apical tubulin ring (ATR) that colocalises with markers of the conoid in related apicomplexan parasites. This tubulin-containing structure was presumed to be lost in Plasmodium despite its crucial role in motility and invasion in other apicomplexans. Here, U-ExM reveals that a divergent and considerably reduced form of the conoid is actually conserved in Plasmodium species. An expansion microscopy study of Plasmodium species, parasites that cause malaria, unveils novel cytoskeletal features including a remnant conoid - a tubulin structure that was presumed to be lost in these organisms despite its crucial role in host cell invasion by related apicomplexan parasites.
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影响因子：
3.3
作者：
Backer CB;Gutzman JH;Pearson CG;Cheeseman IM
通讯作者：
Cheeseman IM
影响因子：
13.6
作者：
Le Guennec, Maeva;Klena, Nikolai;Guichard, Paul
通讯作者：
Guichard, Paul
影响因子：
2.7
作者：
Hirai, Makoto;Arai, Meiji;Matsuoka, Hiroyuki
通讯作者：
Matsuoka, Hiroyuki
影响因子：
2.4
作者：
Bannister, LH;Hopkins, JM;Mitchell, GH
通讯作者：
Mitchell, GH
DOI：
10.1073/pnas.1708304114
发表时间：
2017-09-12
影响因子：
11.1
作者：
Heine, Joern;Reuss, Matthias;Hell, Stefan W.
通讯作者：
Hell, Stefan W.