THE EFFECT OF AGE ON THE ANTIGEN-PRESENTING MECHANISM IN LIMITING DILUTION PRECURSOR CELL FREQUENCY-ANALYSIS
THE EFFECT OF AGE ON THE ANTIGEN-PRESENTING MECHANISM IN LIMITING DILUTION PRECURSOR CELL FREQUENCY-ANALYSIS
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DOI：
10.1016/0008-8749(84)90184-9
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发表时间：
1984-01-01
影响因子：
4.3
通讯作者：
WALFORD, RL
中科院分区：
文献类型：
--
作者：
EFFROS, RB;WALFORD, RL
用有限稀释法（LDA）比较了幼龄和老龄C57 BL/6小鼠流感病毒感染后自身固有前体细胞毒性T淋巴细胞（pCTL）的频率。在过量的白细胞介素2（IL-2）和抗原呈递细胞（APC）的条件下，来自脾脏的小鼠在年龄匹配的测试，超过2倍的差异，pCTL频率之间发现年轻和年老的动物。如果将抗原呈递给来自年轻小鼠的脾细胞上的老年应答细胞，则两个年龄组之间的pCTL频率没有差异。通过标准LDA，老年小鼠中pCTL频率的明显降低实际上可能是由于老年脾细胞的抗原加工和/或呈递机制的缺陷。我们和其他人先前报道的与年龄相关的缺陷性CTL活性可能至少部分是由于衰老小鼠的抗原呈递机制的缺陷。
Limiting dilution analysis (LDA) was used to compare the intrinsic precursor cytotoxic T lymphocyte (pCTL) frequency for influenza-plus-self in young and old C57BL/6 mice. Under conditions of excess interleukin 2 (IL-2) and antigen-presenting cells (APC) derived from spleens of mice matched in age to those being tested, more than a 2-fold difference in pCTL frequency was found between young and old animals. There was no difference in pCTL frequency between the 2 age groups if antigen was presented to the old responder cells on spleen cells derived from young mice. The apparent decrease in pCTL frequency in old mice by standard LDA may in fact be due to a defect in the antigen processing and/or presentation mechanism of old spleen cells. The age-associated defective CTL activity previously reported by us and by others may be due at least in part to a defect in the antigen presentation mechanism of aging mice.