Percutaneous penetration characteristics and release kinetics of contact allergens encapsulated in ethosomes
Percutaneous penetration characteristics and release kinetics of contact allergens encapsulated in ethosomes
复制标题
DOI：
10.3109/15569527.2010.521220
复制
发表时间：
2011-03-01
影响因子：
1.6
通讯作者：
Nielsen, Jesper Bo
中科院分区：
文献类型：
--
作者：
Madsen, Jakob Torp;Vogel, Stefan;Nielsen, Jesper Bo
目的：探讨接触性变应原在乙醇小体中的释放动力学、经皮渗透和吸收模式以及变应原在表皮和真皮中沉积的变化是否可以解释乙醇体致敏和激发特性的增加。方法：采用透析袋研究变应原的释放动力学，并在选定的时间点采集样本，直至达到平衡。用人皮肤对Franz细胞进行经皮吸收和渗透研究，并在选定的时间点采集受体液体样本。实验在24小时后终止，测量变应原在表皮和真皮中的沉积。结果：与乙醇-水制剂相比，醇体制剂可显著降低两种变应原的释放量。与对照制剂相比，二硝基氯苯的醇体制剂增加了其经皮渗透，但降低了异丁香酚的经皮渗透。同样，对于乙醇和乙醇-水中的两种过敏原，所有其他计算参数都显示出相反的趋势。结论：本研究表明，相同的乙醇体会不同地影响两种过敏原的经皮渗透特性。因此，我们的结果表明，过敏原和载体的每一种组合都需要单独评估。化学物质在不同载体中的透皮渗透、释放动力学和致敏性之间的确切机制关系仍有待阐明。
Objective: The aim of this study was to investigate whether the observed increased sensitizing and elicitation properties following the formulation of selected contact allergens in ethosomes could be explained by a change in release kinetics of the allergens and their pattern of percutaneous penetration and absorption as well as allergen deposition in epidermis and dermis.Methods: Release kinetics were studied using dialysis bags, and samples were taken at selected time points until equilibrium was reached. Percutaneous absorption and penetration were studied using human skin on Franz cells, and receptor fluid samples were taken at selected time points. Experiments were terminated after 24 hours, and deposition of allergen in epidermis and dermis was measured. Maximum flux and lag time were calculated.Results: Ethosome formulation decreased the release of both allergens compared with the ethanol--water formulation. Ethosome formulation of dinitrochlorobenzene increased its percutaneous penetration but reduced the percutaneous penetration of isoeugenol compared with control formulations. Likewise, all other calculated parameters showed an opposite trend for the 2 allergens in ethosomes and ethanol--water.Conclusions: The present study demonstrates that identical ethosomes affect the percutaneous penetration characteristics of 2 allergens differently. Thus, our results indicate that each combination of an allergen and a vehicle needs to be evaluated separately. The exact mechanistic relationship between percutaneous penetration, release kinetics, and allergenicity of chemicals in various vehicles remains to be clarified.