Characterization of omega-3-docosahexaenoic acid-containing molecular species of phospholipids in rainbow trout liver
Characterization of omega-3-docosahexaenoic acid-containing molecular species of phospholipids in rainbow trout liver
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DOI：
10.1021/jf960101
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发表时间：
1996-10-01
影响因子：
6.1
通讯作者：
Claeys, M
中科院分区：
文献类型：
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作者：
Chen, S;Claeys, M
用柱层析法对虹鱼肝中的4种主要磷脂进行了纯化，并用P-31核磁共振、气相色谱、液体二次离子质谱仪、液体二次离子质谱仪与串联质谱仪相结合的方法对四种主要磷脂的种类和分子种类进行了定性和定量鉴定。鲑鱼肝脏磷脂主要由甘油磷脂酰胆碱(GPC；67%)、甘油磷脂酰乙醇胺(GPE；22%)、甘油磷脂酰丝氨酸(GPS；10%)和甘油磷脂酰肌醇(GPI；1.6%)组成；前三类磷脂中22：6 omega-3脂肪酸的含量分别为34%(Gpc)、31%(Gpe)和27%(Gps)，丰富的分子种类是1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine(gpc 16：0-22：6)(磷脂分子种名称见表2)，1-eicosaenoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine(gpe20：1-22：6)，1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine(GPE18：0-22：6)、1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine(GPE18：1-22：6)、1-linoleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine(GPE18：2-22：6)、1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine(GPE16：0-22：6)、1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine(GPS16：0-22：6)、和1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine(全球定位系统18：0-22：6)。鲑鱼肝脏中的GPI缺乏含有22：6 omega-3脂肪酸的分子物种。这项研究清楚地表明，虹鱼肝脏的特征是磷脂中含有非常高的22：6 omega-3脂肪酸分子物种，主要是GPC和GPE。
Four major phospholipids from rainbow trout liver were purified by chromatography, and the phospholipid classes and their molecular species were quantitatively and qualitatively determined by P-31 nuclear magnetic resonance, gas chromatography, liquid secondary ion mass spectrometry, and liquid secondary ion mass spectrometry combined with tandem mass spectrometry. Trout liver phospholipids consist mainly of glycerophosphatidylcholine (GPC; 67%), glycerophosphatidylethanolamine (GPE; 22%), glycerophosphatidylserine (GPS; 10%), and glycerophosphatidylinositol (GPI; 1.6%); the contents of 22:6 omega-3 fatty acid in the first three phospholipid classes were 34% (GPC), 31% (GPE), and 27% (GPS), respectively, and abundant molecular species were 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphocholine (GPC 16:0-22:6) (see Table 2 for phospholipid molecular species designations), 1-eicosaenoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 20: 1-22:6), 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 18:0-22:6), 1-oleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 18:1-22:6), 1-linoleoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 18:2-22:6), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoethanolamine (GPE 16:0-22:6), 1-palmitoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine (GPS 16:0-22:6), and 1-stearoyl-2-docosahexaenoyl-sn-glycero-3-phosphoserine (GPS 18:0-22:6). GPI in trout liver lacks molecular species containing 22:6 omega-3 fatty acid. The study clearly indicates that a very high content of 22:6 omega-3 fatty acid-containing molecular species within phospholipids, mainly within GPC and GPE, is characteristic of rainbow trout liver.