Elevated O3 reduces the fitness of Bemisia tabaci via enhancement of the SA-dependent defense of the tomato plant
Elevated O3 reduces the fitness of Bemisia tabaci via enhancement of the SA-dependent defense of the tomato plant
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升高的 O3 通过增强番茄植株的 SA 依赖性防御来降低烟粉虱的适应性
DOI：
10.1007/s11829-012-9189-0
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发表时间：
2012-09-01
影响因子：
1.6
通讯作者：
Ge, Feng
中科院分区：
文献类型：
--
作者：
Cui, Hongying;Sun, Yucheng;Ge, Feng
研究了O3浓度升高对与烟粉虱生物型B相关的3种不同基因型（野生型、茉莉酸（JA）防御增强基因型（35 S）和JA缺陷基因型（spr2））番茄植株的影响。我们通过实验验证了这一假设，即O3浓度升高往往会降低番茄植株的营养，并增加SA依赖的途径防御和次生代谢产物，从而降低粉虱的种群适合度。结果表明，O3浓度升高降低了3种基因型番茄的可溶性糖和游离氨基酸含量，提高了苯丙氨酸解氨酶活性和水杨酸（SA）积累量，并上调了病程相关蛋白（PR1）的表达。O3浓度升高不影响JA水平在任何三种植物基因型，但它增加了一些次生代谢产物的水平，包括总酚和缩合单宁。O3浓度升高延长了三种植物基因型上饲养的粉虱的发育时间，也降低了35S或野生型植物上饲养的粉虱的繁殖力和内在增长率。这些结果表明，O3浓度升高降低了番茄植株的营养，提高了番茄植株的SA含量、PR mRNA表达和次生代谢，导致烟粉虱在番茄植株上的适合度降低。
The effect of elevated O3 on tomato plants of three different genotypes (wild-type, a jasmonic acid (JA) defense-enhanced genotype (35S) and a JA-deficient genotype (spr2)) grown in association with the whitefly Bemisia tabaci Gennadius biotype B was examined in the field in open-top chambers. We experimentally tested the hypothesis that elevated O3 tends to reduce the nutrition of tomato plants, and to increase the SA-dependent pathway defenses and the secondary metabolites, and therefore decrease the population fitness of the whitefly. The results show that for all three tomato genotypes, elevated O3 reduced the soluble sugars and free amino acids, increased the phenylalanine ammonia-lyase enzyme activity and the accumulated salicylic acid (SA), and up-regulated the pathogenesis-related protein (PR1), which is commonly considered to be the whitefly-resistance gene product involved in SA-dependent defense. Elevated O3 did not affect the JA level in any of the three plant genotypes, but it increased the levels of some secondary metabolites, including total phenolics and condensed tannins. Elevated O3 prolonged the developmental time of whiteflies fed on the three plant genotypes, and it also reduced the fecundity and the intrinsic rate of increase of whiteflies fed on either the 35S or the wild-type plants. These results suggest that elevated O3 reduces the nutrition of tomato plants and enhances their SA content, relative PR mRNA expression and secondary metabolism, resulting in decreased fitness of whiteflies on these tomato plants.