Four distinct secretory pathways serve protein secretion, cell surface growth, and peroxisome biogenesis in the yeast Yarrowia lipolytica
Four distinct secretory pathways serve protein secretion, cell surface growth, and peroxisome biogenesis in the yeast Yarrowia lipolytica
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四种不同的分泌途径服务于解脂耶氏酵母中的蛋白质分泌、细胞表面生长和过氧化物酶体生物合成
DOI：
10.1128/mcb.17.9.5210
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发表时间：
1997
影响因子：
5.3
通讯作者：
R. A. Rachubinski
中科院分区：
文献类型：
--
作者：
V. Titorenko;D. Ogrydziak;R. A. Rachubinski
我们已经鉴定和鉴定了溶脂雅罗维菌酵母的突变体，这些突变体缺乏蛋白质分泌，缺乏从酵母到菌丝体的二态转变能力，以及过氧化物体的生物发生。SEC238、SRP54、PEX1、PEX2、PEX6和PEX9基因的突变会影响蛋白质的分泌，阻止碱性胞外蛋白酶的前体形式离开内质网，并影响过氧化物体的生物发生。突变体sec238A、srp54KO、pex2KO、pex6KO和pex9KO也缺乏从酵母到菌丝体的二态转变，并且只影响菌丝体特有的质膜和细胞壁相关蛋白的输出。SEC238、SRP54、PEX1和PEX6基因的突变阻止或显著延缓了两种过氧酶体膜蛋白Pex2p和Pex16p从内质网进入过氧体膜的过程。PEX5、PEX16和PEX17基因的突变以前被证明是过氧化酶体生物发生所必需的，它们影响菌丝体形式特有的质膜和细胞壁相关蛋白的输出，但不损害Pex2p和Pex16p或分泌蛋白从内质网中的输出。对这些突变体的生化分析证明，在酵母和菌丝体的生长模式以及过氧化物体的生物发生过程中，存在四种不同的分泌途径，它们为分泌、质膜和细胞壁的合成提供蛋白质。其中至少有两条分泌途径在内质网水平上存在分歧，这两条途径参与了蛋白质向外部介质的输出和为组装过氧化体膜而运送蛋白质的过程。
We have identified and characterized mutants of the yeast Yarrowia lipolytica that are deficient in protein secretion, in the ability to undergo dimorphic transition from the yeast to the mycelial form, and in peroxisome biogenesis. Mutations in the SEC238, SRP54, PEX1, PEX2, PEX6, and PEX9 genes affect protein secretion, prevent the exit of the precursor form of alkaline extracellular protease from the endoplasmic reticulum, and compromise peroxisome biogenesis. The mutants sec238A, srp54KO, pex2KO, pex6KO, and pex9KO are also deficient in the dimorphic transition from the yeast to the mycelial form and are affected in the export of only plasma membrane and cell wall-associated proteins specific for the mycelial form. Mutations in the SEC238, SRP54, PEX1, and PEX6 genes prevent or significantly delay the exit of two peroxisomal membrane proteins, Pex2p and Pex16p, from the endoplasmic reticulum en route to the peroxisomal membrane. Mutations in the PEX5, PEX16, and PEX17 genes, which have previously been shown to be essential for peroxisome biogenesis, affect the export of plasma membrane and cell wall-associated proteins specific for the mycelial form but do not impair exit from the endoplasmic reticulum of either Pex2p and Pex16p or of proteins destined for secretion. Biochemical analyses of these mutants provide evidence for the existence of four distinct secretory pathways that serve to deliver proteins for secretion, plasma membrane and cell wall synthesis during yeast and mycelial modes of growth, and peroxisome biogenesis. At least two of these secretory pathways, which are involved in the export of proteins to the external medium and in the delivery of proteins for assembly of the peroxisomal membrane, diverge at the level of the endoplasmic reticulum.
DOI：
10.1073/pnas.86.18.6935
发表时间：
1989-09-01
影响因子：
11.1
作者：
ABEIJON, C;ORLEAN, P;HIRSCHBERG, CB
通讯作者：
HIRSCHBERG, CB
通过酵母分泌途径的多肽转运分析。
影响因子：
--
作者：
Franzusoff,A;Rothblatt,J;Schekman,R
通讯作者：
Schekman,R
甲醇、油酸和 D-丙氨酸在博伊丁假丝酵母中诱导的过氧化物酶体的代谢功能各不相同，但具有共同的整合膜蛋白。
影响因子：
4
作者：
Goodman,JM;Trapp,SB;Hwang,H;Veenhuis,M
通讯作者：
Veenhuis,M