Antibiotic Resistance of LACTOBACILLUS Strains
Antibiotic Resistance of LACTOBACILLUS Strains
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DOI：
10.1007/s00284-019-01769-7
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发表时间：
2019-12-01
影响因子：
2.6
通讯作者：
Yarullina, Dina R.
中科院分区：
文献类型：
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作者：
Anisimova, Elizaveta A.;Yarullina, Dina R.
该研究提供了20株乳杆菌属菌株的抗生素抗性的表型和分子分析，其中包括11株新分离的发酵植物材料。纸片扩散法结果显示，90%的乳酸杆菌对克林霉素敏感，95%的乳酸杆菌对四环素、红霉素、利福平敏感。发现氨苄青霉素和氯霉素抑制本研究中使用的所有细菌。绝大多数测试菌株显示对万古霉素、环丙沙星和氨基糖苷类的表型耐药。大多数乳酸杆菌菌株对头孢噻肟、头孢曲松和头孢唑啉的最低抑菌浓度（MIC）较高，因此被认为对头孢菌素类抗生素具有耐药性。所有菌株均表现出多重耐药。耐药基因的发生与表型耐药相关，但表型敏感菌株除外，这些菌株含有四环素（tetK，tetL）和红霉素（ermB，mefA）耐药基因。万古霉素耐药的vanX基因是乳杆菌中最常见的（75%的菌株），但环丙沙星耐药的parC基因的发生是零星的（20%的菌株）。我们的研究结果主要证明了乳杆菌对氨基糖苷类抗生素耐药性的内在本质，尽管在20%的受试菌株中发现了链霉素aadA和aadE酶修饰基因。超广谱β-内酰胺酶（ESBL）的发生是未知的乳酸杆菌，但我们的研究结果显示，blaTEM基因在80%的菌株，而blaSHV和blaOXA-1基因的频率较低（分别为20%和15%的菌株）。
The study provides phenotypic and molecular analyses of the antibiotic resistance in 20 Lactobacillus strains including 11 strains newly isolated from fermented plant material. According to the results of disc diffusion method, 90% of tested lactobacilli demonstrated sensitivity to clindamycin and 95% of strains were susceptible to tetracycline, erythromycin, and rifampicin. Ampicillin and chloramphenicol were found to inhibit all bacteria used in this study. The vast majority of tested strains revealed phenotypic resistance to vancomycin, ciprofloxacin, and aminoglycosides. Most of Lactobacillus strains showed high minimum inhibitory concentrations (MICs) of cefotaxime, ceftriaxone, and cefazolin and therefore were considered resistant to cephalosporins. All the strains exhibited multidrug resistance. The occurrence of resistance genes was associated with phenotypic resistance, with the exception of phenotypically susceptible strains that contained genes for tetracycline (tetK, tetL) and erythromycin (ermB, mefA) resistance. The vanX gene for vancomycin resistance was among the most frequently identified among the lactobacilli (75% of strains), but the occurrence of the parC gene for ciprofloxacin resistance was sporadic (20% of strains). Our results mainly evidence the intrinsic nature of the resistance to aminoglycosides in lactobacilli, though genes for enzymatic modification of streptomycin aadA and aadE were found in 20% of tested strains. The occurrence of extended spectrum beta-lactamases (ESBL) was unknown in Lactobacillus, but our results revealed the blaTEM gene in 80% of strains, whereas blaSHV and blaOXA-1 genes were less frequent (20% and 15% of strains, respectively).